Mouse Anti-ARHGAP39 Recombinant Antibody (H-2) (CBMAB-A3498-YC)

Provided herein is a Mouse monoclonal antibody against Human Rho GTPase Activating Protein 39. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ARHGAP39 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

H-2

Antibody Isotype

IgG2b, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 426-674 mapping within an internal region of ARHGAP39 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Rho GTPase Activating Protein 39

Introduction

ARHGAP39 (Rho GTPase Activating Protein 39) is a protein coding gene. Among its related pathways are Developmental Biology and Signaling by Robo receptor.

Entrez Gene ID

80728

UniProt ID

Q9C0H5

Alternative Names

Rho GTPase Activating Protein 39; Rho GTPase-Activating Protein 39; RhoGAP93B Homolog (Drosophila); CrossGAP Homolog (Drosophila); RhoGAP93B Homolog; CrossGAP Homolog; KIAA1688; CrGAP; Vilse;

Biological Process

Postsynapse organization Source: SynGO
Regulation of small GTPase mediated signal transduction Source: Reactome
Signal transduction Source: InterPro

Cellular Location

Nucleus

References

Howell, M., Delorme-Walker, V., Welch, C., Pathak, R., Hahn, K. M., & DerMardirossian, C. (2021). Mechanistic of Rac and Cdc42 synchronization at the cell edge by ARHGAP39-dependent signaling nodules and the impact on protrusion dynamics. bioRxiv.

Colpo, M. M. (2019). Effects of Varying Insulin Concentration Treatments following Insulin Receptor Knockdown on the Growth Regulating RhoGAP, Arhgap39 (Doctoral dissertation, Ohio University).

Nowak, F. V. (2018). Porf-2= Arhgap39= Vilse: a pivotal role in neurodevelopment, learning and memory. Eneuro, 5(5).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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