Mouse Anti-ARHGEF10 Recombinant Antibody (6G5) (CBMAB-A0466-LY)

The product is antibody recognizes ARHGEF10. The antibody 6G5 immunoassay techniques such as: WB, ELISA.
See all ARHGEF10 antibodies

Published Data

Fig.1 Cell contraction and activation of RhoA by various deletion mutants of ARHGEF10 in HEK293T cells.

Fig.2 Cell contraction induced by ARHGEF10 N in HEK293T cells.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

6G5

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

ARHGEF10 (NP_055444, 792 a.a. ~ 890 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Rho Guanine Nucleotide Exchange Factor 10

Introduction

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. [provided by RefSeq]

Entrez Gene ID

9639

UniProt ID

O15013

Alternative Names

DKFZp686H0726; GEF10; MGC131664

Function

May play a role in developmental myelination of peripheral nerves.

Biological Process

Actin cytoskeleton organization Source: GO_Central
Activation of GTPase activity Source: BHF-UCL
Centrosome duplication Source: BHF-UCL
Mitotic spindle assembly Source: BHF-UCL
Myelination in peripheral nervous system Source: BHF-UCL
Positive regulation of stress fiber assembly Source: BHF-UCL
Regulation of Rho protein signal transduction Source: InterPro

Cellular Location

Centrosome; Cytosol

Involvement in disease

Slowed nerve conduction velocity (SNCV): Affected individuals present a reduction in nerve conduction velocities without any clinical signs of peripheral or central nervous system dysfunction. SNCV inheritance is autosomal dominant.

PTM

Methylated at Gln-1338 by N6AMT1.

References

Khan, A., Ni, W., Lopez‐Giraldez, F., Kluger, M. S., Pober, J. S., & Pierce, R. W. (2021). Tumor necrosis factor‐induced ArhGEF10 selectively activates RhoB contributing to human microvascular endothelial cell tight junction disruption. The FASEB Journal, 35(6), e21627.

Joseph, J., Radulovich, N., Wang, T., Raghavan, V., Zhu, C. Q., & Tsao, M. S. (2020). Rho guanine nucleotide exchange factor ARHGEF10 is a putative tumor suppressor in pancreatic ductal adenocarcinoma. Oncogene, 39(2), 308-321.

Shibata, S., Teshima, Y., Niimi, K., & Inagaki, S. (2019). Involvement of ARHGEF10, GEF for RhoA, in Rab6/Rab8-mediating membrane traffic. Small GTPases, 10(3), 169-177.

Lu, D., Liou, H. C., Liao, H. M., Chen, C. H., Gau, S. S. F., & Fu, W. M. (2017). ARHGEF10 Knockout Mice Exhibit Autism‐Like Behavior and Altered Pain Sensation. The FASEB Journal, 31, 812-12.

Lu, D. H., Hsu, C. C., Huang, S. W., Tu, H. J., Huang, T. F., Liou, H. C., ... & Gau, S. F. (2017). ARHGEF 10 knockout inhibits platelet aggregation and protects mice from thrombus formation. Journal of Thrombosis and Haemostasis, 15(10), 2053-2064.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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