Mouse Anti-ARHGEF11 Recombinant Antibody (EG236) (CBMAB-EN263-LY)

Name: Mouse Anti-ARHGEF11 Recombinant Antibody (EG236)
SKU: CBMAB-EN263-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ARHGEF11 Recombinant Antibody (EG236) CBMAB-EN263-LY in Immunoblot

Immunoblot analyses of pMLC2 and MLC2 levels A375M2 for siRNA knock-down of ARHGEF1, ARHGEF2 and ARHGEF11, respectively. ...View More

Maiques, O., Fanshawe, B., Crosas-Molist, E., Rodriguez-Hernandez, I., Volpe, A., Cantelli, G., ... & Fruhwirth, G. O. (2021). A preclinical pipeline to evaluate migrastatics as therapeutic agents in metastatic melanoma. British Journal of Cancer, 125(5), 699-713.

Mouse Anti-ARHGEF11 Recombinant Antibody (EG236) CBMAB-EN263-LY in IF

Knockdown of ARHGEF11 attenuates EMT, cell cycle progression and induces cell apoptosis via β-catenin-dependent way. (A) Correlation between the β-catenin and ARHGEF11 expression from TCGA HCC dataset analyzed by online CHIPbase. (B) β-Catenin expression from whole-cell lysates (up panel), the cytoplasmic and cell nuclear lysates (below panel) detected by western blot. (C) β-Catenin expression determined via immunofluorescence. ...View More

Du, J., Zhu, Z., Xu, L., Chen, X., Li, X., Lan, T., ... & Zeng, Y. (2020). ARHGEF11 promotes proliferation and epithelial-mesenchymal transition of hepatocellular carcinoma through activation of β-catenin pathway. Aging (Albany NY), 12(20), 20235.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG236

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1223-1522 mapping at the C-terminus of PDZ-RhoGEF of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Rho Guanine Nucleotide Exchange Factor 11

Introduction

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	9826
Mouse	213498
Rat	78966

UniProt ID

Human	O15085
Mouse	Q68FM7
Rat	Q9ES67

Alternative Names

Rho Guanine Nucleotide Exchange Factor 11; Rho Guanine Nucleotide Exchange Factor (GEF) 11; PDZ-RHOGEF; Glutamate Transporter EAAT4-Associated Protein 48; RhoA-Specific Guanine Nucleotide Exchange Factor; Rho Guanine Exchange Factor (GEF) 11; RhoGEF Glutamate Transport Modulator; KIAA0380; GTRAP48;

Function

May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Involved in neurotrophin-induced neurite outgrowth.

Biological Process

Actin cytoskeleton organization Source: UniProtKB
Establishment of cell polarity Source: UniProtKB
G protein-coupled receptor signaling pathway Source: MGI
Positive regulation of apoptotic process Source: Reactome
Positive regulation of GTPase activity Source: GOC
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of cell growth Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: UniProtKB
Striated muscle contraction Source: UniProtKB

Cellular Location

Cytoplasm; Membrane. Translocated to the membrane upon stimulation.

PTM

Phosphorylated by MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14).
Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex when previously phosphorylated by MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14), leading to its degradation, thereby restricting RhoA activity and facilitating growth cone spreading and neurite outgrowth.

Mizuki, Y., Takaki, M., Sakamoto, S., Okamoto, S., Kishimoto, M., Okahisa, Y., ... & Yamada, N. (2017). Human rho guanine nucleotide exchange factor 11 (ARHGEF11) regulates dendritic morphogenesis. International journal of molecular sciences, 18(1), 67.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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