Mouse Anti-ARHGEF11 Recombinant Antibody (EG236) (CBMAB-EN263-LY)
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Datasheet
Target
Q & As
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Protocols
Associated Products
Basic Information
Host Animal
Mouse
Clone
EG236
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 1223-1522 mapping at the C-terminus of PDZ-RhoGEF of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
| Application | Note |
| WB | 1:100-1:1,000 |
| IP | 1-2 µg per 100-500 µg of total protein (1 ml of cell lysate) |
| IF(ICC) | 1:50-1:500 |
| ELISA | 1:100-1:1,000 |
Formulations & Storage [For reference only, actual COA shall prevail!]
Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation
Target
Full Name
Rho Guanine Nucleotide Exchange Factor 11
Introduction
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Alternative Names
Rho Guanine Nucleotide Exchange Factor 11; Rho Guanine Nucleotide Exchange Factor (GEF) 11; PDZ-RHOGEF; Glutamate Transporter EAAT4-Associated Protein 48; RhoA-Specific Guanine Nucleotide Exchange Factor; Rho Guanine Exchange Factor (GEF) 11; RhoGEF Glutamate Transport Modulator; KIAA0380; GTRAP48;
Function
May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Involved in neurotrophin-induced neurite outgrowth.
Biological Process
Actin cytoskeleton organization Source: UniProtKB
Establishment of cell polarity Source: UniProtKB
G protein-coupled receptor signaling pathway Source: MGI
Positive regulation of apoptotic process Source: Reactome
Positive regulation of GTPase activity Source: GOC
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of cell growth Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: UniProtKB
Striated muscle contraction Source: UniProtKB
Establishment of cell polarity Source: UniProtKB
G protein-coupled receptor signaling pathway Source: MGI
Positive regulation of apoptotic process Source: Reactome
Positive regulation of GTPase activity Source: GOC
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of cell growth Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: UniProtKB
Striated muscle contraction Source: UniProtKB
Cellular Location
Cytoplasm; Membrane. Translocated to the membrane upon stimulation.
PTM
Phosphorylated by MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14).
Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex when previously phosphorylated by MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14), leading to its degradation, thereby restricting RhoA activity and facilitating growth cone spreading and neurite outgrowth.
Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex when previously phosphorylated by MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14), leading to its degradation, thereby restricting RhoA activity and facilitating growth cone spreading and neurite outgrowth.
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Please try the standard protocols which include: protocols, troubleshooting and guide.
For research use only. Not intended for any clinical use.
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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