Mouse Anti-ARHGEF16 Recombinant Antibody (5A11F8) (CBMAB-R0419-CN)

This product is a Mouse antibody that recognizes ARHGEF16. The antibody 5A11F8 can be used for immunoassay techniques such as: ELISA, IHC, WB.
See all ARHGEF16 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat, Cattle, Dog, Horse, Pig

Clone

5A11F8

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 683-708 at the C-terminus of ARHGEF16 of human origin.

Specificity

Human, Mouse, Rat, Cattle, Dog, Horse, Pig

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Target

Full Name

Rho Guanine Nucleotide Exchange Factor 16

Introduction

Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	27237
Mouse	230972
Rat	687105

UniProt ID

Human	Q5VV41
Mouse	Q3U5C8
Rat	B2GUW2

Alternative Names

Rho Guanine Nucleotide Exchange Factor 16; Rho Guanine Nucleotide Exchange Factor (GEF) 16; Ephexin-4; NBR; Rho Guanine Exchange Factor (GEF) 16; Putative Neuroblastoma Protein; EPHEXIN4; GEF16;

Function

Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.

Biological Process

Activation of GTPase activity Source: BHF-UCL
Cell chemotaxis Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
Positive regulation of apoptotic process Source: Reactome
Positive regulation of protein localization to plasma membrane Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome

Cellular Location

Cytoplasm

References

Yu, B., Xu, L., Chen, L., Wang, Y., Jiang, H., Wang, Y., ... & Zhai, Z. (2020). FYN is required for ARHGEF16 to promote proliferation and migration in colon cancer cells. Cell death & disease, 11(8), 1-14.

Huang, D., Wang, Y., Xu, L., Chen, L., Cheng, M., Shi, W., ... & Luo, S. (2018). GLI2 promotes cell proliferation and migration through transcriptional activation of ARHGEF16 in human glioma cells. Journal of Experimental & Clinical Cancer Research, 37(1), 1-17.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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