Mouse Anti-ARHGEF18 Recombinant Antibody (8H6) (CBMAB-A0469-LY)

Name: Mouse Anti-ARHGEF18 Recombinant Antibody (8H6)
SKU: CBMAB-A0469-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

8H6

Application

WB, ELISA

Immunogen

ARHGEF18 (AAH08016.1, 1 a.a. ~ 89 a.a) full-length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

rho/rac guanine nucleotide exchange factor (GEF) 18

Introduction

Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Entrez Gene ID

23370

UniProt ID

Q6ZSZ5

Alternative Names

KIAA0521; MGC15913; P114-RhoGEF

Function

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950).

Biological Process

Actin cytoskeleton organization Source: MGI
G protein-coupled receptor signaling pathway Source: Reactome
Negative regulation of stress fiber assembly Source: ARUK-UCL
Positive regulation of apoptotic process Source: Reactome
Protein localization to cell-cell junction Source: ARUK-UCL
Regulation of cell shape Source: MGI
Regulation of Rho protein signal transduction Source: GO_Central
Regulation of small GTPase mediated signal transduction Source: Reactome
Small GTPase mediated signal transduction Source: MGI
Transforming growth factor beta receptor signaling pathway Source: Reactome

Cellular Location

Cytoskeleton; Cytoplasm; Cell membrane; Apical cell membrane. In unactivated eosinophils, distributed around the cell periphery in the perimembranous region (PubMed:29601110). In activated eosinophils, relocates to the tip of the nucleopod, a membrane structure formed during activation when the nucleus moves to one end of the cell, and is also concentrated in membrane protrusions at the opposite end of the cell (PubMed:29601110). Localizes to the apical cell membrane in epithelial cells (PubMed:22006950).

Involvement in disease

Retinitis pigmentosa 78 (RP78): A form of retinitis pigmentosa, a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP78 inheritance is autosomal recessive.

Li, D., Sun, Y., Kong, X., Luan, C., Yu, Y., Chen, F., & Chen, P. (2018). Association between a single nucleotide polymorphism in the 3-UTR of ARHGEF18 and the risk of nonidiopathic pulmonary arterial hypertension in Chinese population. Disease markers, 2018.

Turton, K. B., Wilkerson, E. M., Hebert, A. S., Fogerty, F. J., Schira, H. M., Botros, F. E., ... & Mosher, D. F. (2018). Expression of novel “LOCGEF” isoforms of ARHGEF18 in eosinophils. Journal of leukocyte biology, 104(1), 135-145.

Arno, G., Carss, K. J., Hull, S., Zihni, C., Robson, A. G., Fiorentino, A., ... & Kazmi, R. (2017). Biallelic mutation of ARHGEF18, involved in the determination of epithelial apicobasal polarity, causes adult-onset retinal degeneration. The American Journal of Human Genetics, 100(2), 334-342.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-ARHGEF18 Recombinant Antibody (GT1972) (CAT#: CBMAB-P0071-YC)

Isotype control

For research use only. Not intended for any clinical use.

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