Mouse Anti-ARHGEF4 Recombinant Antibody (ARHGEF-08) (CBMAB-A3529-YC)

Provided herein is a Mouse monoclonal antibody against Human Rho Guanine Nucleotide Exchange Factor 4. The antibody can be used for immunoassay techniques, such as IHC-Fr.
See all ARHGEF4 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

ARHGEF-08

Antibody Isotype

IgG1

Application

WB, FC

Basic Information

Immunogen

Recombinant fragment of human ARHGEF4 (amino acids 143-271).

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:2,000
FC	1-4 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

15mM sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 143-271

Target

Full Name

Rho Guanine Nucleotide Exchange Factor 4

Introduction

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent sig

Entrez Gene ID

50649

UniProt ID

Q9NR80

Alternative Names

Rho Guanine Nucleotide Exchange Factor 4; APC-Stimulated Guanine Nucleotide Exchange Factor 1; Rho Guanine Nucleotide Exchange Factor (GEF) 4; ASEF1; ASEF; APC-Stimulated Guanine Nucleotide Exchange Factor; KIAA1112; GEF4; STM6;

Function

Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.

Biological Process

Filopodium assembly Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
Intracellular signal transduction Source: InterPro
Lamellipodium assembly Source: UniProtKB
Positive regulation of apoptotic process Source: Reactome
Regulation of small GTPase mediated signal transduction Source: Reactome

Cellular Location

Isoform 3: Ruffle membrane; Cytoplasm. Associated with membrane ruffles.

References

Lee, K., Yoo, K. S., Park, Y. S., & Kim, H. K. (2021). Activity of Arhgef4 is modulated through Staufen1 in neurons. Neuroscience Letters, 756, 135962.

Yoo, K. S., Lee, K., Lee, Y. S., Oh, W. J., & Kim, H. K. (2020). Rho guanine nucleotide exchange factor 4 (Arhgef4) deficiency enhances spatial and object recognition memory. Experimental Neurobiology, 29(5), 334.

Zhang, X., Sun, L., Chen, W., Wu, S., Li, Y., Li, X., ... & Xu, A. (2019). ARHGEF4-mediates the actin cytoskeleton reorganization of hepatic stellate cells in 3-dimensional collagen matrices. Cell adhesion & migration, 13(1), 170-182.

Taniuchi, K., Furihata, M., Naganuma, S., & Saibara, T. (2018). ARHGEF4 predicts poor prognosis and promotes cell invasion by influencing ERK1/2 and GSK-3α/β signaling in pancreatic cancer. International journal of oncology, 53(5), 2224-2240.

Mangolini, M., Gasparoli, L., Virely, C., Edwards, D., Bartram, J., Goulden, N., ... & Williams, O. (2017). Targeting ARHGEF4 in cancer. Klinische Pädiatrie, 229(03), A5.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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