Mouse Anti-ARID4B Recombinant Antibody (CBYY-0025) (CBMAB-0025-YY)

This product is mouse antibody that recognizes ARID4B. The antibody CBYY-0025 can be used for immunoassay techniques such as: WB, IP, IF, ELISA
See all ARID4B antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBYY-0025

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 867-952 mapping within an internal region of BRCAA1 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

N-Terminus

Target

Full Name

AT-Rich Interaction Domain 4B

Introduction

This gene encodes a protein with sequence similarity to retinoblastoma-binding protein-1. The encoded protein is a subunit of the histone deacetylase-dependant SIN3A transcriptional corepressor complex, which functions in diverse cellular processes including proliferation, differentiation, apoptosis, oncogenesis, and cell fate determination. The gene product is recognized by IgG antiBovinedy isolated from a breast cancer patient and appears to be a molecular marker associated with a broad range of human malignancies. Alternate transcriptional splice variants encoding different isoforMouse have been characterized.

Entrez Gene ID

51742

UniProt ID

Q4LE39

Alternative Names

AT-Rich Interaction Domain 4B; Histone Deacetylase Complex Subunit SAP180; AT Rich Interactive Domain 4B (RBP1-Like); Breast Cancer-Associated Antigen BRCAA1; Retinoblastoma-Binding Protein 1-Like 1; 180 KDa Sin3-Associated Polypeptide; ARID Domain-Containing Protein 4B; Sin3-Associated Polypeptide P180; RBBP1L1; RBP1L1; BRCAA1;

Function

Acts as a transcriptional repressor (PubMed:12724404).
May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (PubMed:12724404).
Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A. Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR (androgen receptor) and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier. Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor (By similarity).

Biological Process

Chromatin organization Source: UniProtKB-KW
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus; Cytoplasm. Cytoplasmic in breast cancer cells.

References

Luo, S. M., Tsai, W. C., Tsai, C. K., Chen, Y., & Hueng, D. Y. (2021). ARID4B Knockdown Suppresses PI3K/AKT Signaling and Induces Apoptosis in Human Glioma Cells. OncoTargets and therapy, 14, 1843.

Ren, J., Yao, H., Hu, W., Perrett, S., Gong, W., & Feng, Y. (2021). Structural basis for the DNA-binding activity of human ARID4B Tudor domain. Journal of Biological Chemistry, 296.

Ofori, S. (2021). Rational Design of Small Molecule Disruptors of Protein-Protein Interactions: PD-1/PD-L1; c-MYC, ARID4B.

Miao, X., Xi, Z., Zhang, Y., Li, Z., Huang, L., Xin, T., ... & Wang, T. (2020). Circ-SMARCA5 suppresses colorectal cancer progression via downregulating miR-39-3p and upregulating ARID4B. Digestive and Liver Disease, 52(12), 1494-1502.

Wang, R., Yu, Z., Chen, F., Liao, C., Wang, Q., & Huang, X. (2018). Overexpression of ARID4B predicts poor survival in patients with hepatocellular carcinoma. Human pathology, 73, 114-121.

Liang, Y. K., Han, Z. D., Lu, J. M., Liu, Z. Z., Zhuo, Y. J., Zhu, X. J., ... & Zhong, W. D. (2018). Downregulation of ARID4A and ARID4B promote tumor progression and directly regulated by microRNA‐30d in patient with prostate cancer. Journal of cellular biochemistry, 119(9), 7245-7255.

Wu, R. C., Zeng, Y., Chen, Y. F., Lanz, R. B., & Wu, M. Y. (2017). Temporal‐Spatial Establishment of Initial Niche for the Primary Spermatogonial Stem Cell Formation Is Determined by an ARID4B Regulatory Network. Stem Cells, 35(6), 1554-1565.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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