Mouse Anti-ARL14 Recombinant Antibody (2C8) (CBMAB-A0450-LY)

The product is antibody recognizes ARF7. The antibody 2C8 immunoassay techniques such as: sELISA, ELISA.
See all ARL14 antibodies

Summary

Host Animal

Mouse

Specificity

Human

Clone

2C8

Antibody Isotype

IgG1, λ

Application

ELISA

Basic Information

Immunogen

ARF7 (AAH34354, 1 a.a. ~ 192 a.a) full-length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG1, λ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

ADP Ribosylation Factor Like GTPase 14

Entrez Gene ID

80117

UniProt ID

Q8N4G2

Alternative Names

ARF7; FLJ22595

Function

GTPase that recruits MYO1E to MHC class II-containing vesicles via the effector protein ARL14EP and hence controls the movement of these vesicles along the actin cytoskeleton in dendritic cells.

Biological Process

Intracellular protein transport Source: GO_Central
Vesicle-mediated transport Source: GO_Central

Cellular Location

Cytoplasmic vesicle. Colocalizes with MHC II-containing cytoplasmic vesicles.

References

Yang, F., Li, T., Peng, Z., Liu, Y., & Guo, Y. (2020). The amphipathic helices of Arfrp1 and Arl14 are sufficient to determine subcellular localizations. Journal of Biological Chemistry, 295(49), 16643-16654.

Guo, F., Yuan, D., Zhang, J., Zhang, H., Wang, C., Zhu, L., ... & Shao, C. (2019). Silencing of ARL14 gene induces lung adenocarcinoma cells to a dormant state. Frontiers in cell and developmental biology, 7, 238.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

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