Mouse Anti-ARL3 Recombinant Antibody (1C6) (CBMAB-A3588-YC)

Name: Mouse Anti-ARL3 Recombinant Antibody (1C6)
SKU: CBMAB-A3588-YC
Rating: 5 (1 reviews)

See all ARL3 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1C6

Application

WB, IHC

Immunogen

Human recombinant protein fragment corresponding to amino acids 2-182 of human ARL3 produced in E. coli

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
IHC	1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ADP Ribosylation Factor Like GTPase 3

Introduction

ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity.

Entrez Gene ID

403

UniProt ID

P36405

Alternative Names

ADP Ribosylation Factor Like GTPase 3; ADP-Ribosylation Factor-Like 3; ARFL3; ADP-Ribosylation Factor-Like Protein 3; ARF-Like 3;

Function

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP) (PubMed:16525022, PubMed:18588884).
Required for normal cytokinesis and cilia signaling (PubMed:22085962).
Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins to the cilium, including myristoylated NPHP3 and prenylated INPP5E (PubMed:30269812).
Targets NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (PubMed:22085962).
Required for PKD1:PKD2 complex targeting from the trans-Golgi network to the cilium (By similarity).

Biological Process

Cilium assembly Source: UniProtKB
Golgi to plasma membrane transport Source: UniProtKB
Intraciliary transport Source: Ensembl
Kidney development Source: UniProtKB
Mitotic cytokinesis Source: UniProtKB
Photoreceptor cell development Source: UniProtKB
Positive regulation of microtubule polymerization Source: InterPro
Post-Golgi vesicle-mediated transport Source: MGI
Protein localization to ciliary membrane Source: UniProtKB
Protein localization to cilium Source: UniProtKB
Small GTPase mediated signal transduction Source: UniProtKB
Smoothened signaling pathway Source: Ensembl

Cellular Location

Spindle; Centrosome; Golgi apparatus membrane; Cytoplasm; Nucleus; Cilium. Detected predominantly in the photoreceptor connecting cilium. Present on the mitotic spindle. Centrosome-associated throughout the cell cycle. Not detected to interphase microtubules.

Involvement in disease

Joubert syndrome 35 (JBTS35): A form of Joubert syndrome, a disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy, renal disease, liver fibrosis, and polydactyly. JBTS35 inheritance is autosomal recessive.
Retinitis pigmentosa 83 (RP83): An autosomal dominant form of retinitis pigmentosa, a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.

ElMaghloob, Y., Sot, B., McIlwraith, M. J., Garcia, E., Yelland, T., & Ismail, S. (2021). ARL3 activation requires the co-GEF BART and

Holtan, J. P., Teigen, K., Aukrust, I., Bragadóttir, R., & Houge, G. (2019). Dominant ARL3-related retinitis pigmentosa. Ophthalmic genetics, 40(2), 124-128.

Luo, G., Sun, Y., Feng, R., Zhao, Q., & Wen, T. (2018). ARL3 subcellular localization and its suspected role in autophagy. Biochimie, 154, 187-193.

Alkanderi, S., Molinari, E., Shaheen, R., Elmaghloob, Y., Stephen, L. A., Sammut, V., ... & Sayer, J. A. (2018). ARL3 mutations cause Joubert syndrome by disrupting ciliary protein composition. The American Journal of Human Genetics, 103(4), 612-620.

Hanke-Gogokhia, C., Frederick, J. M., Zhang, H., & Baehr, W. (2018). Binary function of ARL3-GTP revealed by gene knockouts. Retinal Degenerative Diseases, 317-325.

Ivanova, A. A., Caspary, T., Seyfried, N. T., Duong, D. M., West, A. B., Liu, Z., & Kahn, R. A. (2017). Biochemical characterization of purified mammalian ARL13B protein indicates that it is an atypical GTPase and ARL3 guanine nucleotide exchange factor (GEF). Journal of Biological Chemistry, 292(26), 11091-11108.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ARL3 Recombinant Antibody (1C6)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-ARL3 Recombinant Antibody (5E6) (CAT#: CBMAB-A3595-YC)

Mouse Anti-ARL3 Recombinant Antibody (2A9) (CAT#: CBMAB-A3589-YC)

Mouse Anti-ARL3 Recombinant Antibody (3A10) (CAT#: CBMAB-A3591-YC)

Mouse Anti-ARL3 Recombinant Antibody (2G7) (CAT#: CBMAB-A3590-YC)

Mouse Anti-ARL3 Recombinant Antibody (3E1) (CAT#: CBMAB-A3594-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry