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Mouse Anti-ARPC2 Recombinant Antibody (F-5) (CBMAB-P0072-YC)

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Published Data
Mouse Anti-ARPC2 Recombinant Antibody (F-5) CBMAB-P0072-YC in WB
Mouse Anti-ARPC2 Recombinant Antibody (F-5) CBMAB-P0072-YC in WB
Disappearance of the Arp2/3 complex was detected by western blot analysis (n = 3). ...View More
Choi, J., Lee, Y. J., Yoon, Y. J., Kim, C. H., Park, S. J., Kim, S. Y., ... & Kwon, B. M. (2019). Pimozide suppresses cancer cell migration and tumor metastasis through binding to ARPC2, a subunit of the Arp2/3 complex. Cancer science, 110(12), 3788-3801.
Datasheet
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Basic Information

Host Animal
Mouse
Clone
F-5
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 1-300 of p34-ARC of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
actin related protein 2/3 complex, subunit 2, 34kDa
Introduction
ARPC2 is one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.
Entrez Gene ID
Human10109
Mouse76709
Rat301511
UniProt ID
HumanO15144
MouseQ9CVB6
RatP85970
Alternative Names
ARC34; PNAS-139; PRO2446; p34-Arc
Function
Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079).
The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079).
Seems to contact the mother actin filament (PubMed:9230079).
In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947).
The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
Biological Process
Actin filament polymerization Source: InterPro
Actin polymerization-dependent cell motility Source: UniProtKB
Arp2/3 complex-mediated actin nucleation Source: FlyBase
Ephrin receptor signaling pathway Source: Reactome
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Membrane organization Source: Reactome
Positive regulation of lamellipodium assembly Source: UniProtKB
Positive regulation of substrate adhesion-dependent cell spreading Source: Ensembl
Cellular Location
Cytoskeleton; Nucleus; Cell projection; Synaptosome

Cheng, Z., Wei, W., Wu, Z., Wang, J., Ding, X., Sheng, Y., ... & Wu, Q. (2019). ARPC2 promotes breast cancer proliferation and metastasis. Oncology reports, 41(6), 3189-3200.

Chen, P., Yue, X., Xiong, H., Lu, X., & Ji, Z. (2019). RBM3 upregulates ARPC2 by binding the 3'UTR and contributes to breast cancer progression. International journal of oncology, 54(4), 1387-1397.

Choi, J., Lee, Y. J., Yoon, Y. J., Kim, C. H., Park, S. J., Kim, S. Y., ... & Kwon, B. M. (2019). Pimozide suppresses cancer cell migration and tumor metastasis through binding to ARPC2, a subunit of the Arp2/3 complex. Cancer science, 110(12), 3788-3801.

Al-Zeer, M. A., Dutkiewicz, M., von Hacht, A., Kreuzmann, D., Röhrs, V., & Kurreck, J. (2019). Alternatively spliced variants of the 5’-UTR of the ARPC2 mRNA regulate translation by an internal ribosome entry site (IRES) harboring a guanine-quadruplex motif. RNA biology, 16(11), 1622-1632.

Yoon, Y. J., Han, Y. M., Choi, J., Lee, Y. J., Yun, J., Lee, S. K., ... & Kwon, B. M. (2019). Benproperine, an ARPC2 inhibitor, suppresses cancer cell migration and tumor metastasis. Biochemical pharmacology, 163, 46-59.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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