Mouse Anti-ARPC2 Recombinant Antibody (F-5) (CBMAB-P0072-YC)

Provided herein is a Mouse monoclonal antibody against Human Actin Related Protein 2/3 Complex Subunit 2. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ARPC2 antibodies

Published Data

Mouse Anti-ARPC2 Recombinant Antibody (F-5) CBMAB-P0072-YC in WB

Disappearance of the Arp2/3 complex was detected by western blot analysis (n = 3). ...View More

Choi, J., Lee, Y. J., Yoon, Y. J., Kim, C. H., Park, S. J., Kim, S. Y., ... & Kwon, B. M. (2019). Pimozide suppresses cancer cell migration and tumor metastasis through binding to ARPC2, a subunit of the Arp2/3 complex. Cancer science, 110(12), 3788-3801.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

F-5

Antibody Isotype

IgG2a, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 1-300 of p34-ARC of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

actin related protein 2/3 complex, subunit 2, 34kDa

Introduction

ARPC2 is one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

Entrez Gene ID

Human	10109
Mouse	76709
Rat	301511

UniProt ID

Human	O15144
Mouse	Q9CVB6
Rat	P85970

Alternative Names

ARC34; PNAS-139; PRO2446; p34-Arc

Function

Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079).
The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079).
Seems to contact the mother actin filament (PubMed:9230079).
In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947).
The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).

Biological Process

Actin filament polymerization Source: InterPro
Actin polymerization-dependent cell motility Source: UniProtKB
Arp2/3 complex-mediated actin nucleation Source: FlyBase
Ephrin receptor signaling pathway Source: Reactome
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Membrane organization Source: Reactome
Positive regulation of lamellipodium assembly Source: UniProtKB
Positive regulation of substrate adhesion-dependent cell spreading Source: Ensembl

Cellular Location

Cytoskeleton; Nucleus; Cell projection; Synaptosome

References

Cheng, Z., Wei, W., Wu, Z., Wang, J., Ding, X., Sheng, Y., ... & Wu, Q. (2019). ARPC2 promotes breast cancer proliferation and metastasis. Oncology reports, 41(6), 3189-3200.

Chen, P., Yue, X., Xiong, H., Lu, X., & Ji, Z. (2019). RBM3 upregulates ARPC2 by binding the 3'UTR and contributes to breast cancer progression. International journal of oncology, 54(4), 1387-1397.

Al-Zeer, M. A., Dutkiewicz, M., von Hacht, A., Kreuzmann, D., Röhrs, V., & Kurreck, J. (2019). Alternatively spliced variants of the 5’-UTR of the ARPC2 mRNA regulate translation by an internal ribosome entry site (IRES) harboring a guanine-quadruplex motif. RNA biology, 16(11), 1622-1632.

Yoon, Y. J., Han, Y. M., Choi, J., Lee, Y. J., Yun, J., Lee, S. K., ... & Kwon, B. M. (2019). Benproperine, an ARPC2 inhibitor, suppresses cancer cell migration and tumor metastasis. Biochemical pharmacology, 163, 46-59.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ARPC2 Recombinant Antibody (CBYC-P020)

Mouse Anti-ARPC2 Recombinant Antibody (5C8)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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