Mouse Anti-ASCC1 Recombinant Antibody (1E2) (CBMAB-A3732-YC)

Provided herein is a Mouse monoclonal antibody against Human Activating Signal Cointegrator 1 Complex Subunit 1. The antibody can be used for immunoassay techniques, such as FC, IF, WB.
See all ASCC1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

1E2

Antibody Isotype

IgG1

Application

FC, IF, WB

Basic Information

Immunogen

Full length human recombinant protein of human ASCC1 produced in HEK293T cell.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	1:100
FC	1:100
WB	1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Activating Signal Cointegrator 1 Complex Subunit 1

Introduction

ASCC1 is a subunit of the activating signal cointegrator 1 (ASC-1) complex. The ASC-1 complex is a transcriptional coactivator that plays an important role in gene transactivation by multiple transcription factors including activating protein 1 (AP-1), nu

Entrez Gene ID

51008

UniProt ID

Q8N9N2

Alternative Names

Activating Signal Cointegrator 1 Complex Subunit 1; ASC-1 Complex Subunit P50; Trip4 Complex Subunit P50; ASC1p50; CGI-18; SMABF2; P50;

Function

Plays a role in DNA damage repair as component of the ASCC complex (PubMed:29997253).
Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed:12077347).
In cells responding to gastrin-activated paracrine signals, it is involved in the induction of SERPINB2 expression by gastrin. May also play a role in the development of neuromuscular junction.

Biological Process

DNA dealkylation involved in DNA repair Source: Reactome
Regulation of transcription, DNA-templated Source: MGI

Cellular Location

Nucleus; Nucleus speckle. Colocalizes with PRPF8 in nuclear speckles in the absence of DNA damage.

Involvement in disease

Barrett esophagus (BE): A condition characterized by a metaplastic change in which normal esophageal squamous epithelium is replaced by a columnar and intestinal-type epithelium. Patients with Barrett esophagus have an increased risk of esophageal adenocarcinoma. The main cause of Barrett esophagus is gastroesophageal reflux. The retrograde movement of acid and bile salts from the stomach into the esophagus causes prolonged injury to the esophageal epithelium and induces chronic esophagitis, which in turn is believed to trigger the pathologic changes.
Spinal muscular atrophy with congenital bone fractures 2 (SMABF2): An autosomal recessive neuromuscular disorder characterized by prenatal-onset spinal muscular atrophy, multiple congenital contractures consistent with arthrogryposis multiplex congenita, respiratory distress, and congenital bone fractures.

References

Cho, H. W., Jin, H. S., & Eom, Y. B. (2020). Association between non-Caucasian-specific ASCC1 gene polymorphism and osteoporosis and obesity in Korean postmenopausal women. Journal of Bone and Mineral Metabolism, 38(6), 868-877.

Giuffrida, M. G., Mastromoro, G., Guida, V., Truglio, M., Fabbretti, M., Torres, B., ... & Pizzuti, A. (2020). A new case of SMABF2 diagnosed in stillbirth expands the prenatal presentation and mutational spectrum of ASCC1. American Journal of Medical Genetics Part A, 182(3), 508-512.

Böhm, J., Malfatti, E., Oates, E., Jones, K., Brochier, G., Boland, A., ... & Laporte, J. (2019). Novel ASCC1 mutations causing prenatal-onset muscle weakness with arthrogryposis and congenital bone fractures. Journal of medical genetics, 56(9), 617-621.

Soll, J. M., Brickner, J. R., Mudge, M. C., & Mosammaparast, N. (2018). RNA ligase-like domain in activating signal cointegrator 1 complex subunit 1 (ASCC1) regulates ASCC complex function during alkylation damage. Journal of Biological Chemistry, 293(35), 13524-13533.

Oliveira, J., Martins, M., Pinto Leite, R., Sousa, M., & Santos, R. (2017). The new neuromuscular disease related with defects in the ASC‐1 complex: Report of a second case confirms ASCC1 involvement. Clinical genetics, 92(4), 434-439.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ASCC1 Recombinant Antibody (6G5)

Mouse Anti-ASCC1 Recombinant Antibody (1H10)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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