Mouse Anti-ASS1 Recombinant Antibody (CF037) (CBMAB-FT447LY)

Name: Mouse Anti-ASS1 Recombinant Antibody (CF037)
SKU: CBMAB-FT447LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CF037

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 373-400 at the C-terminus of ASS1 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Argininosuccinate Synthase 1

Introduction

The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2012]

Entrez Gene ID

Human	445
Mouse	11898
Rat	25698

UniProt ID

Human	P00966
Mouse	P16460
Rat	P09034

Alternative Names

Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1

Function

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.

Biological Process

Acute-phase response Source: Ensembl
Aging Source: Ensembl
Arginine biosynthetic process Source: UniProtKB
Argininosuccinate metabolic process Source: BHF-UCL
Aspartate metabolic process Source: BHF-UCL
Cellular response to amine stimulus Source: Ensembl
Cellular response to amino acid stimulus Source: Ensembl
Cellular response to ammonium ion Source: Ensembl
Cellular response to cAMP Source: Ensembl
Cellular response to dexamethasone stimulus Source: Ensembl
Cellular response to glucagon stimulus Source: Ensembl
Cellular response to interferon-gamma Source: Ensembl
Cellular response to laminar fluid shear stress Source: BHF-UCL
Cellular response to lipopolysaccharide Source: Ensembl
Cellular response to oleic acid Source: Ensembl
Cellular response to tumor necrosis factor Source: Ensembl
Circadian rhythm Source: UniProtKB
Citrulline metabolic process Source: BHF-UCL
Diaphragm development Source: Ensembl
Kidney development Source: Ensembl
Liver development Source: Ensembl
Midgut development Source: Ensembl
Negative regulation of leukocyte cell-cell adhesion Source: BHF-UCL
Positive regulation of nitric oxide biosynthetic process Source: BHF-UCL
Response to drug Source: Ensembl
Response to estradiol Source: Ensembl
Response to growth hormone Source: Ensembl
Response to mycotoxin Source: Ensembl
Response to nutrient Source: Ensembl
Response to zinc ion Source: Ensembl
Urea cycle Source: UniProtKB

Cellular Location

Cytosol

Involvement in disease

Citrullinemia 1 (CTLN1): The classic form of citrullinemia, an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. It is a disorder of the urea cycle, usually manifesting in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder form can develop later in childhood or adulthood.

PTM

Acetylated by CLOCK in a circadian manner which negatively regulates its enzyme activity. Deacetylated by histone deacetylases.

Li, J. M., Yang, D. C., Oldham, J., Linderholm, A., Zhang, J., Liu, J., ... & Chen, C. H. (2021). Therapeutic targeting of argininosuccinate synthase 1 (ASS1)-deficient pulmonary fibrosis. Molecular Therapy, 29(4), 1487-1500.

Giatromanolaki, A., Harris, A. L., & Koukourakis, M. I. (2021). The prognostic and therapeutic implications of distinct patterns of argininosuccinate synthase 1 (ASS1) and arginase-2 (ARG2) expression by cancer cells and tumor stroma in non-small-cell lung cancer. Cancer & metabolism, 9(1), 1-10.

Kim, S., Lee, M., Song, Y., Lee, S. Y., Choi, I., Park, I. S., ... & Seo, H. R. (2021). Argininosuccinate synthase 1 suppresses tumor progression through activation of PERK/eIF2α/ATF4/CHOP axis in hepatocellular carcinoma. Journal of Experimental & Clinical Cancer Research, 40(1), 1-18.

Lehrke, H. D., Van Treeck, B. J., Allende, D., Denham, L. J., Gonzalez, R. S., Moreira, R. K., ... & Graham, R. P. (2020). Does Argininosuccinate Synthase 1 (ASS1) immunohistochemistry predict an increased risk of hemorrhage for hepatocellular adenomas?. Applied Immunohistochemistry & Molecular Morphology, 28(6), 464-470.

Nault, J. C., Couchy, G., Caruso, S., Meunier, L., Caruana, L., Letouzé, E., ... & Zucman‐Rossi, J. (2018). Argininosuccinate synthase 1 and periportal gene expression in sonic hedgehog hepatocellular adenomas. Hepatology, 68(3), 964-976.

Henriet, E., Abou Hammoud, A., Dupuy, J. W., Dartigues, B., Ezzoukry, Z., Dugot‐Senant, N., ... & Saltel, F. (2017). Argininosuccinate synthase 1 (ASS1): a marker of unclassified hepatocellular adenoma and high bleeding risk. Hepatology, 66(6), 2016-2028.

Ohshima, K., Nojima, S., Tahara, S., Kurashige, M., Hori, Y., Hagiwara, K., ... & Morii, E. (2017). Argininosuccinate synthase 1-deficiency enhances the cell sensitivity to arginine through decreased DEPTOR expression in endometrial cancer. Scientific reports, 7(1), 1-14.

Miyamoto, T., Lo, P. H. Y., Saichi, N., Ueda, K., Hirata, M., Tanikawa, C., & Matsuda, K. (2017). Argininosuccinate synthase 1 is an intrinsic Akt repressor transactivated by p53. Science advances, 3(5), e1603204.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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