Mouse Anti-ATAT1 Recombinant Antibody (1) (V2LY-0725-LY328)

Name: Mouse Anti-ATAT1 Recombinant Antibody (1)
SKU: V2LY-0725-LY328
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

ELISA, WB, IF, IP

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IF	1:50-1:200
IP	1:200-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

alpha tubulin acetyltransferase 1

Entrez Gene ID

79969

UniProt ID

Q5SQI0

Alternative Names

TAT; MEC17; C6orf134; Nbla00487; alpha-TAT; alpha-TAT1

Function

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.

Biological Process

Alpha-tubulin acetylation
Cilium assembly
Dentate gyrus development
Microtubule cytoskeleton organization
Neuron development
NLRP3 inflammasome complex assembly
Positive regulation of NLRP3 inflammasome complex assembly
Regulation of fat cell differentiation
Regulation of microtubule cytoskeleton organization
Response to mechanical stimulus
Response to pain
Spermatogenesis

Cellular Location

Cytoplasm;
Membrane, clathrin-coated pit;
Cell junction, focal adhesion;
Cell projection, axon;
Cytoplasm, cytoskeleton;
Cytoplasm, cytoskeleton, spindle.

PTM

Autoacetylation strongly increases tubulin acetylation.

Rahimi, A. M., Cai, M., Kılıҫ, I., Kazerouni, Z. B., Tapia Contreras, C., & Hoyer-Fender, S. (2021). Expression of α-Tubulin Acetyltransferase 1 and Tubulin Acetylation as Selective Forces in Cell Competition. Cells, 10(2), 390.

Yanai, R., Yamashita, Y., Umezu, K., Hiradate, Y., Hara, K., & Tanemura, K. (2020). Expression and localization of alpha-tubulin N-acetyltransferase 1 in the reproductive system of male mice. Journal of Reproduction and Development.

Even, A., Morelli, G., Broix, L., Scaramuzzino, C., Turchetto, S., Gladwyn-Ng, I., ... & Nguyen, L. (2019). ATAT1-enriched vesicles promote microtubule acetylation via axonal transport. Science advances, 5(12), eaax2705.

Li, L., Jayabal, S., Ghorbani, M., Legault, L. M., McGraw, S., Watt, A. J., & Yang, X. J. (2019). ATAT1 regulates forebrain development and stress-induced tubulin hyperacetylation. Cellular and Molecular Life Sciences, 76(18), 3621-3640.

Zhou, H., Cheng, X., Xu, X., Jiang, T., Zhou, H., Sheng, Q., & Nie, Z. (2018). Cloning, expression profiling, and acetylation identification of alpha‐tubulin N‐acetyltransferase 1 from Bombyx mori. Archives of insect biochemistry and physiology, 98(3), e21463.

Nekooki-Machida, Y., Nakakura, T., Nishijima, Y., Tanaka, H., Arisawa, K., Kiuchi, Y., ... & Hagiwara, H. (2018). Dynamic localization of α-tubulin acetyltransferase ATAT1 through the cell cycle in human fibroblastic KD cells. Medical molecular morphology, 51(4), 217-226.

Morelli, G., Even, A., Gladwyn-Ng, I., Le Bail, R., Shilian, M., Godin, J. D., ... & Nguyen, L. (2018). p27kip1 modulates axonal transport by regulating α-tubulin acetyltransferase 1 stability. Cell reports, 23(8), 2429-2442.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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