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Mouse Anti-ATF6B Recombinant Antibody (CBYY-C0764) (CBMAB-C2200-YY)

This product is mouse antibody that recognizes ATF6B. The antibody CBYY-C0764 can be used for immunoassay techniques such as: ELISA, IF, WB
See all ATF6B antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-C0764
Antibody Isotype
IgG2a, κ
Application
WB, IF, IP, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 2-88 of ATF-6β of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Activating Transcription Factor 6 Beta
Entrez Gene ID
1388
UniProt ID
Q99941
Function
Cyclic AMP-dependent transcription factor ATF-6 beta: Precursor of the transcription factor form (Processed cyclic AMP-dependent transcription factor ATF-6 beta), which is embedded in the endoplasmic reticulum membrane (PubMed:11256944).
Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR) (PubMed:11256944).
Processed cyclic AMP-dependent transcription factor ATF-6 beta: Transcription factor that acts in the unfolded protein response (UPR) pathway by activating UPR target genes induced during ER stress (PubMed:11256944).
Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAATN9CCAC[GA]-3') when NF-Y is bound to ERSE (PubMed:11256944).
Biological Process
ATF6-mediated unfolded protein response Source: ParkinsonsUK-UCL
Endoplasmic reticulum unfolded protein response Source: GO_Central
Negative regulation of ATF6-mediated unfolded protein response Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction Source: ProtInc
Cellular Location
Endoplasmic reticulum membrane
Processed cyclic AMP-dependent transcription factor ATF-6 beta: Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
Topology
Cytoplasmic: 2-396 aa
Helical: 397-417 aa
Lumenal: 418-703 aa
PTM
N-glycosylated.
During unfolded protein response, a fragment of approximately 60 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is probably performed sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) proteases.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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