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Mouse Anti-ATG4D Recombinant Antibody (15.4.1) (CBMAB-A3934-YC)

Provided herein is a Mouse monoclonal antibody against Human Autophagy Related 4D Cysteine Peptidase. The antibody can be used for immunoassay techniques, such as ELISA, ICC, IF, WB.
See all ATG4D antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
15.4.1
Antibody Isotype
IgG1, κ
Application
IF, WB

Basic Information

Immunogen
Recombinant protein corresponding to Atg4D of human origin.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Autophagy Related 4D Cysteine Peptidase
Introduction
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, a
Entrez Gene ID
84971
UniProt ID
Q86TL0
Alternative Names
Autophagy Related 4D Cysteine Peptidase; Autophagy-Related Cysteine Endopeptidase 4; Autophagy-Related Protein 4 Homolog D; AUT-Like 4 Cysteine Endopeptidase; Autophagin-4; APG4D; AUTL4; AUT-Like 4, Cysteine Endopeptidase (S. Cerevisiae); ATG4 Autophagy R
Function
Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability.
Biological Process
Apoptotic process Source: UniProtKB-KW
Autophagy Source: UniProtKB-KW
Protein transport Source: UniProtKB-KW
Proteolysis Source: UniProtKB
Cellular Location
Cysteine protease ATG4D: Cytoplasm
Cysteine protease ATG4D, mitochondrial: Mitochondrion matrix; Cytoplasm. Imported into mitochondrial matrix after cleavage by CASP3 during oxidative stress and cell death.
PTM
Cleaved by CASP3 during apoptosis which leads to increased activity. The cleavage by CASP3 reveals a cryptic mitochondrial targeting sequence immediately downstream of their canonical caspase cleavage sites which leads to mitochondrial import of the protein.

Sha, Y., Liu, W., Wei, X., Zhu, X., Tang, B., Zhang, X., ... & Wang, X. (2021). Pathogenic variants of ATG4D in infertile men with non‐obstructive azoospermia identified using whole‐exome sequencing. Clinical Genetics.

Zhao, J. Y., Li, X. Y., Liu, T. D., Liang, B., Huang, Y., & Li, W. (2021). Silencing of ATG4D suppressed proliferation and enhanced cisplatin-induced apoptosis in hepatocellular carcinoma through Akt/Caspase-3 pathway. Molecular and Cellular Biochemistry, 1-7.

Syrjä, P., Palviainen, M., Jokinen, T., Kyöstilä, K., Lohi, H., Roosje, P., ... & Eskelinen, E. L. (2020). Altered Basal Autophagy Affects Extracellular Vesicle Release in Cells of Lagotto Romagnolo Dogs With a Variant ATG4D. Veterinary pathology, 57(6), 926-935.

Fu, Z., Luo, W., Wang, J., Peng, T., Sun, G., Shi, J., ... & Zhang, B. (2017). Malat1 activates autophagy and promotes cell proliferation by sponging miR-101 and upregulating STMN1, RAB5A and ATG4D expression in glioma. Biochemical and biophysical research communications, 492(3), 480-486.

El Andaloussi, A., Habib, S., Soylemes, G., Laknaur, A., Elhusseini, H., Al-Hendy, A., & Ismail, N. (2017). Defective expression of ATG4D abrogates autophagy and promotes growth in human uterine fibroids. Cell death discovery, 3(1), 1-9.

Wang, Y., Sun, H., Song, J., Yao, G., Sun, H., & Ge, Z. (2017). MiR-139-5p protect against myocardial ischemia and reperfusion (I/R) injury by targeting autophagy-related 4D and inhibiting AMPK/mTOR/ULK1 pathway. International journal of clinical and experimental pathology, 10(9), 10140.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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