Mouse Anti-ATG4D Recombinant Antibody (15.4.1) (CBMAB-A3934-YC)

Name: Mouse Anti-ATG4D Recombinant Antibody (15.4.1)
SKU: CBMAB-A3934-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

15.4.1

Application

IF, WB

Immunogen

Recombinant protein corresponding to Atg4D of human origin.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Autophagy Related 4D Cysteine Peptidase

Introduction

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, a

Entrez Gene ID

84971

UniProt ID

Q86TL0

Alternative Names

Autophagy Related 4D Cysteine Peptidase; Autophagy-Related Cysteine Endopeptidase 4; Autophagy-Related Protein 4 Homolog D; AUT-Like 4 Cysteine Endopeptidase; Autophagin-4; APG4D; AUTL4; AUT-Like 4, Cysteine Endopeptidase (S. Cerevisiae); ATG4 Autophagy R

Function

Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability.

Biological Process

Apoptotic process Source: UniProtKB-KW
Autophagy Source: UniProtKB-KW
Protein transport Source: UniProtKB-KW
Proteolysis Source: UniProtKB

Cellular Location

Cysteine protease ATG4D: Cytoplasm
Cysteine protease ATG4D, mitochondrial: Mitochondrion matrix; Cytoplasm. Imported into mitochondrial matrix after cleavage by CASP3 during oxidative stress and cell death.

PTM

Cleaved by CASP3 during apoptosis which leads to increased activity. The cleavage by CASP3 reveals a cryptic mitochondrial targeting sequence immediately downstream of their canonical caspase cleavage sites which leads to mitochondrial import of the protein.

Sha, Y., Liu, W., Wei, X., Zhu, X., Tang, B., Zhang, X., ... & Wang, X. (2021). Pathogenic variants of ATG4D in infertile men with non‐obstructive azoospermia identified using whole‐exome sequencing. Clinical Genetics.

Zhao, J. Y., Li, X. Y., Liu, T. D., Liang, B., Huang, Y., & Li, W. (2021). Silencing of ATG4D suppressed proliferation and enhanced cisplatin-induced apoptosis in hepatocellular carcinoma through Akt/Caspase-3 pathway. Molecular and Cellular Biochemistry, 1-7.

Syrjä, P., Palviainen, M., Jokinen, T., Kyöstilä, K., Lohi, H., Roosje, P., ... & Eskelinen, E. L. (2020). Altered Basal Autophagy Affects Extracellular Vesicle Release in Cells of Lagotto Romagnolo Dogs With a Variant ATG4D. Veterinary pathology, 57(6), 926-935.

Fu, Z., Luo, W., Wang, J., Peng, T., Sun, G., Shi, J., ... & Zhang, B. (2017). Malat1 activates autophagy and promotes cell proliferation by sponging miR-101 and upregulating STMN1, RAB5A and ATG4D expression in glioma. Biochemical and biophysical research communications, 492(3), 480-486.

El Andaloussi, A., Habib, S., Soylemes, G., Laknaur, A., Elhusseini, H., Al-Hendy, A., & Ismail, N. (2017). Defective expression of ATG4D abrogates autophagy and promotes growth in human uterine fibroids. Cell death discovery, 3(1), 1-9.

Wang, Y., Sun, H., Song, J., Yao, G., Sun, H., & Ge, Z. (2017). MiR-139-5p protect against myocardial ischemia and reperfusion (I/R) injury by targeting autophagy-related 4D and inhibiting AMPK/mTOR/ULK1 pathway. International journal of clinical and experimental pathology, 10(9), 10140.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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