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Rabbit Anti-ATG5 Recombinant Antibody (CBYC-A859) (CBMAB-A3947-YC)

Provided herein is a Rabbit monoclonal antibody against Human Autophagy Related 5. The antibody can be used for immunoassay techniques, such as ICC, IF, IHC-P, IP, WB.
See all ATG5 antibodies
Published Data
Rabbit Anti-ATG5 Recombinant Antibody (CBYC-A859) (CBMAB-A3947-YC) in WB
Western blotting analysis shows the expression of ATG5 in HCC cells cocultured with Exo-Ts alone or cotransfected with miR-205-5p mimics while showing the rescue effect of ATG5 overexpression vector (oe-ATG5) on the autophagic inhibition of miR-205-5p mimics. ...View More
Wang, X., Dong, F., Wang, Y., Wei, H., Li, T., & Li, J. (2023). Exosomal circTGFBR2 promotes hepatocellular carcinoma progression via enhancing ATG5 mediated protective autophagy.
Rabbit Anti-ATG5 Recombinant Antibody (CBYC-A859) (CBMAB-A3947-YC) in WB
Representative cropped blots with relative immunoblotting analysis of ATG5 protein levels in EC treated for 24h with 20 ng/mL IL-6 (IL-6), with 100 µg/mL PCSK9i (PCSK9i) or pre-treated for 8h with 100 µg/mL PCSK9i before being exposed to 20 ng/mL IL-6 for 24h (PCSK9i+IL-6). ...View More
D'Onofrio, N., Prattichizzo, F., Marfella, R., Sardu, C., Martino, E., Scisciola, L., ... & Balestrieri, M. L. (2023). SIRT3 mediates the effects of PCSK9 inhibitors on inflammation, autophagy, and oxidative stress in endothelial cells. Theranostics, 13(2), 531.

Summary

Host Animal
Rabbit
Specificity
Human, Monkey
Clone
CBYC-A859
Antibody Isotype
IgG
Application
IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Gly232 of human Atg5 protein.
Specificity
Human, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
10mM HEPES, pH 7.5, 0.15M NaCl, 0.1mg/mL BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Autophagy Related 5
Introduction
Autophagy Related 5, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitocho
Entrez Gene ID
Human9474
Mouse11793
Rat365601
UniProt ID
HumanQ9H1Y0
MouseQ99J83
RatQ3MQ06
Alternative Names
Autophagy Related 5; Apoptosis-Specific Protein; APG5-LIKE; APG5L; ASP; ATG5 Autophagy Related 5 Homolog (S. Cerevisiae); APG5 (Autophagy 5, S. Cerevisiae)-Like; APG5 Autophagy 5-Like (S. Cerevisiae);
Function
Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.
(Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747).
Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established (PubMed:19666601).
Biological Process
Aggrephagy Source: ParkinsonsUK-UCL
Antigen processing and presentation of endogenous antigen Source: Ensembl
Apoptotic process Source: UniProtKB-KW
Autophagosome assembly Source: UniProtKB
Autophagy Source: UniProtKB
Autophagy of mitochondrion Source: GO_Central
Autophagy of nucleus Source: GO_Central
Blood vessel remodeling Source: Ensembl
Cellular response to nitrogen starvation Source: GO_Central
Cellular response to nitrosative stress Source: Ensembl
C-terminal protein lipidation Source: GO_Central
Heart contraction Source: Ensembl
Macroautophagy Source: Reactome
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of cell death Source: MGI
Negative regulation of histone H4-K16 acetylation Source: Ensembl
Negative regulation of phagocytosis Source: Ensembl
Negative regulation of protein ubiquitination Source: Ensembl
Negative regulation of reactive oxygen species metabolic process Source: Ensembl
Negative stranded viral RNA replication Source: Ensembl
Negative thymic T cell selection Source: Ensembl
Otolith development Source: Ensembl
Positive regulation by symbiont of host autophagy Source: UniProtKB
Positive regulation of mucus secretion Source: Ensembl
Post-translational protein modification Source: UniProtKB
Protein lipidation involved in autophagosome assembly Source: Ensembl
Regulation of cilium assembly Source: UniProtKB
Regulation of cytokine production involved in immune response Source: Ensembl
Regulation of release of sequestered calcium ion into cytosol Source: Ensembl
Response to drug Source: Ensembl
Response to fungus Source: Ensembl
Vasodilation Source: Ensembl
Ventricular cardiac muscle cell development Source: Ensembl
Cellular Location
Cytoplasm; Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
Involvement in disease
Spinocerebellar ataxia, autosomal recessive, 25 (SCAR25): A form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR25 patients manifest delayed psychomotor development with delayed walking, truncal ataxia, dysmetria, and nystagmus, Cerebellar hypoplasia is seen on brain imaging.
PTM
Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
Acetylated by EP300.

Huang, Q., Liu, Y., Zhang, S., Yap, Y. T., Li, W., Zhang, D., ... & Zhang, Z. (2020). Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice. Autophagy, 1-15.

Wang, C. Z., Yan, G. X., Dong, D. S., Xin, H., & Liu, Z. Y. (2019). LncRNA-ATB promotes autophagy by activating Yes-associated protein and inducing autophagy-related protein 5 expression in hepatocellular carcinoma. World journal of gastroenterology, 25(35), 5310.

Li, N. A., Fan, X., Wang, X., Deng, H., Zhang, K., Zhang, X., ... & Liu, Z. (2019). Autophagy-related 5 gene rs510432 polymorphism is associated with hepatocellular carcinoma in patients with chronic hepatitis B virus infection. Immunological investigations, 48(4), 378-391.

Görgülü, K., Diakopoulos, K. N., Ai, J., Schoeps, B., Kabacaoglu, D., Karpathaki, A. F., ... & Algül, H. (2019). Levels of the autophagy-related 5 protein affect progression and metastasis of pancreatic tumors in mice. Gastroenterology, 156(1), 203-217.

Xi, Z., Si, J., & Nan, J. (2019). LncRNA MALAT1 potentiates autophagy‑associated cisplatin resistance by regulating the microRNA‑30b/autophagy‑related gene 5 axis in gastric cancer. International journal of oncology, 54(1), 239-248.

Sprott, D., Poitz, D. M., Korovina, I., Ziogas, A., Phieler, J., Chatzigeorgiou, A., ... & Klotzsche-von Ameln, A. (2019). Endothelial-specific deficiency of ATG5 (autophagy protein 5) attenuates ischemia-related angiogenesis. Arteriosclerosis, thrombosis, and vascular biology, 39(6), 1137-1148.

Guo, H., Sadoul, R., & Gibbings, D. (2018). Autophagy-independent effects of autophagy-related-5 (Atg5) on exosome production and metastasis. Molecular & cellular oncology, 5(3), e1445941.

Ye, X., Zhou, X. J., & Zhang, H. (2018). Exploring the role of autophagy-related gene 5 (ATG5) yields important insights into autophagy in autoimmune/autoinflammatory diseases. Frontiers in immunology, 9, 2334.

Hu, Z. Y., Chen, B., Zhang, J. P., & Ma, Y. Y. (2017). Up-regulation of autophagy-related gene 5 (ATG5) protects dopaminergic neurons in a zebrafish model of Parkinson's disease. Journal of Biological Chemistry, 292(44), 18062-18074.

Xu, J., Xia, L., Shang, Q., Du, J., Zhu, D., Wang, Y., ... & Xing, Q. (2017). A variant of the autophagy-related 5 gene is associated with child cerebral palsy. Frontiers in cellular neuroscience, 11, 407.

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For research use only. Not intended for any clinical use.

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