Mouse Anti-ATOH7 Recombinant Antibody (1A5) (CBMAB-A0598-LY)

The product is antibody recognizes ATOH7. The antibody 1A5 immunoassay techniques such as: WB, ELISA.
See all ATOH7 antibodies

Published Data

Mouse Anti-ATOH7 Recombinant Antibody (1A5) (CBMAB-A0598-LY) in WB

Western blots of EMSA nuclear extracts probed with ATOH7 or E47 antibodies show similar levels of these proteins. A low level of endogenous E47 is present in untransfected lanes. ...View More

Prasov, L., Masud, T., Khaliq, S., Mehdi, S. Q., Abid, A., Oliver, E. R., ... & Glaser, T. (2012). ATOH7 mutations cause autosomal recessive persistent hyperplasia of the primary vitreous. Human molecular genetics, 21(16), 3681-3694.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1A5

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

ATOH7 (NP_660161, 53 a.a. ~ 99 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

atonal homolog 7 (Drosophila)

Introduction

ATOH7 is a member of the family of basic helix-loop-helix (bHLH) proteins with similarity to Drosophila 'atonal,' a proneural bHLH gene that controls photoreceptor development (Brown et al., 2002 [PubMed 11889557]).[supplied by OMIM

Entrez Gene ID

220202

UniProt ID

Q8N100

Alternative Names

Math5; bHLHa13

Function

Transcription factor that positively regulates the determination of retinal ganglion cell fate and formation of the optic nerve and retino-hypothalamic tract (By similarity).
Required for retinal circadian rhythm photoentrainment (By similarity).

Biological Process

Circadian rhythm Source: Ensembl
Entrainment of circadian clock by photoperiod Source: UniProtKB
Neural retina development Source: MGI
Neuron differentiation Source: GO_Central
Optic nerve development Source: MGI
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

Involvement in disease

Persistent hyperplastic primary vitreous, autosomal recessive (PHPVAR): The disease is caused by variants affecting the gene represented in this entry. A 6.5 kb deletion that spans a remote cis regulatory element 20 kb upstream from ATOH7 has been found in PHPVAR patients (PubMed:21441919). A developmental eye malformation associated with microphthalmia, cataract, glaucoma, and congenital retinal non-attachment. It is due to failure of the primary vitreous to regress in utero, resulting in the presence of a retrolental fibrovascular membrane with persistence of the posterior portion of the tunica vasculosa lentis and hyaloid artery. Disease manifestations range from a trivial remnant of hyaloid vessels to a dense fibrovascular mass causing lens opacity and retinal detachment.

References

Brodie-Kommit, J., Clark, B. S., Shi, Q., Shiau, F., Kim, D. W., Langel, J., ... & Hattar, S. (2021). Atoh7-independent specification of retinal ganglion cell identity. Science advances, 7(11), eabe4983.

Atac, D., Koller, S., Hanson, J. V., Feil, S., Tiwari, A., Bahr, A., ... & Berger, W. (2020). Atonal homolog 7 (ATOH7) loss-of-function mutations in predominant bilateral optic nerve hypoplasia. Human molecular genetics, 29(1), 132-148.

Miesfeld, J. B., Ghiasvand, N. M., Marsh-Armstrong, B., Marsh-Armstrong, N., Miller, E. B., Zhang, P., ... & Glaser, T. (2020). The Atoh7 remote enhancer provides transcriptional robustness during retinal ganglion cell development. Proceedings of the National Academy of Sciences, 117(35), 21690-21700.

Kondkar, A. A., Azad, T. A., Almobarak, F. A., Bahabri, I. M., Kalantan, H., Abu-Amero, K. K., & Al-Obeidan, S. A. (2018). Lack of association between variant rs7916697 in ATOH7 and primary open angle glaucoma in a Saudi cohort. Genetics research international, 2018.

Miesfeld, J. B., Moon, M. S., Riesenberg, A. N., Contreras, A. N., Kovall, R. A., & Brown, N. L. (2018). Rbpj direct regulation of Atoh7 transcription in the embryonic mouse retina. Scientific reports, 8(1), 1-13.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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