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Mouse Anti-ATP5PB Recombinant Antibody (9D1BC4) (CBMAB-A4067-YC)

Provided herein is a Mouse monoclonal antibody against Human ATP Synthase Peripheral Stalk-Membrane Subunit B. The antibody can be used for immunoassay techniques, such as WB, IP, IHC-P, ICC, FC, ELISA.
See all ATP5PB antibodies
Published Data
Mouse Anti-ATP5PB Recombinant Antibody (9D1BC4) in WB
Transgenic complementation of ATP synthase deficiency. (A) WB detection of ATP syn-thase subunits in liver and heart homogenates and (B) quantification of the content of the F1-α, Fo-a, Fo-b, and Fo-c subunits relative to the SDH content (SDHA) in the control SHR rats and in the SHR-Tmem70ko/ko,tg/0 (tg/0) or SHR-Tmem70ko/ko,tg/tg (tg/tg) rats derived from SHR-Tmem70tg/tg trans-genic lines 130 and 126. (C) Tissue Tmem70 mRNA levels were analysed by RT-PCR and normalized to HPRT mRNA levels. Data are mean ± SEM, n = 3-6, * p ≤ 0.05, *** p ≤ 0.001. ...View More
Marković, A., Tauchmannová, K., Šimáková, M., Mlejnek, P., Kaplanová, V., Pecina, P., ... & Mráček, T. (2022). Genetic Complementation of ATP Synthase Deficiency Due to Dysfunction of TMEM70 Assembly Factor in Rat. Biomedicines, 10(2), 276.
Mouse Anti-ATP5PB Recombinant Antibody (9D1BC4) in WB
PC12 cells, either untransfected or treated with siRNA(PLCβ1) for 48 h, were stimulated with carbachol or isoproterenol for Gαq or Gαi activation, respectively, or subjected toheat shock or hypo-osmotic stress, followed treatment with MG132 to prevent protein degradation before by lysis and western blotting where n=3, * denotes p values between 0.01 and<0.001 and SE is shown. ...View More
Jackson, L., & Scarlata, S. F. (2022). Activation of Gαq sequesters specific transcripts into Ago2 particles. Biophysical Journal, 121(3), 206a.
Mouse Anti-ATP5PB Recombinant Antibody (9D1BC4) in WB
NDUFA9, ATP5F1, TFAM, MnSOD, and Ferridoxin levels in mitochondrial extract. ...View More
Flax, J., Wilkins, H. M., Miller, R., Griffith, S., Cork, G. K., Qiang, A., ... & Slawson, C. (2020). OGA Inhibition alters energetics and nutrient sensing in Alzheimer's disease cytoplasmic hybrids. Journal of Alzheimer's Disease, 78(4), 1743-1753.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Cattle
Clone
9D1BC4
Antibody Isotype
IgG2b
Application
WB, IHC, ICC, IF, FC

Basic Information

Immunogen
Human ATP5PB.
Specificity
Human, Mouse, Rat, Cattle
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC5 µg/ml
WB1 µg/ml
FC1 µg/ml
IF(ICC)1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATP Synthase Peripheral Stalk-Membrane Subunit B
Introduction
ATP5PB is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked mult
Entrez Gene ID
Human515
Mouse11950
Rat171375
Cattle282701
UniProt ID
HumanP24539
MouseQ9CQQ7
RatP19511
CattleP13619
Alternative Names
ATP Synthase Peripheral Stalk-Membrane Subunit B; ATP Synthase, H+ Transporting, Mitochondrial Fo Complex Subunit B1; ATP5F1; ATP Synthase Proton-Transporting Mitochondrial F(0) Complex Subunit B1; ATP Synthase, H+ Transporting, Mitochondrial F0 Complex,
Function
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements.
Biological Process
ATP biosynthetic process Source: Reactome
ATP synthesis coupled proton transport Source: GO_Central
Cristae formation Source: Reactome
Mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
Substantia nigra development Source: UniProtKB
Cellular Location
Mitochondrion; Mitochondrion inner membrane
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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