Mouse Anti-ATP6V0D2 Recombinant Antibody (7A4) (CBMAB-A0638-LY)

The product is antibody recognizes ATP6V0D2. The antibody 7A4 immunoassay techniques such as: WB, ELISA.
See all ATP6V0D2 antibodies

Published Data

Fig.1 Adrm1 interacts with ATP6v0d2.

Fig.2 Genotyping of mouse genomic DNA using PCR from the tail of 6-week old mice, showing conditional deletion of floxed Atp6ap2 contingent on coinheritance of Myh11-Cre (a).

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

7A4

Antibody Isotype

IgG2b, κ

Application

WB, ELISA

Basic Information

Immunogen

ATP6V0D2 (NP_689778, 238 a.a. ~ 306 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2

Entrez Gene ID

245972

UniProt ID

Q8N8Y2

Alternative Names

ATP6D2; FLJ38708; VMA6

Function

Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity).

Biological Process

Cellular response to amino acid starvation Source: Reactome
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Phagosome acidification Source: Reactome
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Transferrin transport Source: Reactome
Vacuolar acidification Source: GO_Central
Vacuolar transport Source: GO_Central

Cellular Location

Early endosome; Endosome membrane; Extracellular exosome; Lysosomal membrane; Apical plasma membrane; Plasma membrane proton-transporting V-type ATPase complex; Membrane; Phagocytic vesicle membrane; Proton-transporting V-type ATPase, V0 domain; Vacuolar proton-transporting V-type ATPase complex

References

Xia, Y., Liu, N., Xie, X., Bi, G., Ba, H., Li, L., ... & Yang, X. P. (2019). The macrophage-specific V-ATPase subunit ATP6V0D2 restricts inflammasome activation and bacterial infection by facilitating autophagosome-lysosome fusion. Autophagy, 15(6), 960-975.

Yang, J., Guo, F., Yuan, L., Lv, G., Gong, J., & Chen, J. (2019). Elevated expression of the V‐ATPase D2 subunit triggers increased energy metabolite levels in KrasG12D‐driven cancer cells. Journal of cellular biochemistry, 120(7), 11690-11701.

Zhu, X., Zeng, Z., Qiu, D., & Chen, J. (2018). Vγ9Vδ2 T cells inhibit immature dendritic cell transdifferentiation into osteoclasts through downregulation of RANK, c‑Fos and ATP6V0D2. International journal of molecular medicine, 42(4), 2071-2079.

Ayodele, B. A., Mirams, M., Pagel, C. N., & Mackie, E. J. (2017). The vacuolar H+ ATPase V0 subunit D2 is associated with chondrocyte hypertrophy and supports chondrocyte differentiation. Bone reports, 7, 98-107.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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