Mouse Anti-ATP6V1D Recombinant Antibody (3G4) (CBMAB-A4100-YC)

Name: Mouse Anti-ATP6V1D Recombinant Antibody (3G4)
SKU: CBMAB-A4100-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3G4

Application

ELISA, IHC-P, WB

Immunogen

Recombinant protein, ATP6V1D (NP_057078, 69-169 aa) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 69-169

More Infomation

Target

Full Name

ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D

Introduction

ATP6V1D is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zy

Entrez Gene ID

51382

UniProt ID

Q9Y5K8

Alternative Names

ATPase H+ Transporting V1 Subunit D; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump); ATPase, H+ Transporting, Lysosomal 34kDa, V1 Subunit D; V-ATPase 28 KDa Accessory Protein; Vacuolar Proton Pump Subunit D; V-ATPase Subunit D; ATP6M; VATD; H(+

Function

Subunit of the peripheral V1 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity).
May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium.

Biological Process

Cellular response to amino acid starvation Source: Reactome
Cilium assembly Source: UniProtKB
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Neutrophil degranulation Source: Reactome
Phagosome acidification Source: Reactome
Protein localization to cilium Source: UniProtKB
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Transferrin transport Source: Reactome

Cellular Location

Membrane. Localizes to centrosome and the base of the cilium.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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