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Mouse Anti-ATP6V1E1 Recombinant Antibody (4E11) (CBMAB-A4102-YC)

Provided herein is a Mouse monoclonal antibody against Human ATPase H+ Transporting V1 Subunit E1. The antibody can be used for immunoassay techniques, such as ELISA, IF, IHC-P, WB.
See all ATP6V1E1 antibodies
Published Data
Fig. 1 Western blot of ATP6V1E1 and other components of the V1V0-ATPase in equivalent amounts of exosomes produced by wild-type and ATG5−/− MDA-MB-231 to show alterations in levels of specific cargoes in exosomes.
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Fig. 2 Immunofluorescence microscopy of ATP6V1E1 showing its co-localization with the MVB and exosome marker CD63. Arrows indicate co-localization of ATP6V1E1 with CD63. Scale bars, 10 μm.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
4E11
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
ATP6V1E1 (AAH04443, 1 a.a. ~ 226 a.a) full-length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
Introduction
ATP6V1E1 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, z
Entrez Gene ID
529
UniProt ID
P36543
Alternative Names
ATPase H+ Transporting V1 Subunit E1; ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E1; Vacuolar Proton Pump Subunit E 1; V-ATPase 31 KDa Subunit; V-ATPase Subunit E 1; ATP6E2; ATP6E; P31; ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E Isof
Function
Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:32001091).
V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091).
Biological Process
Cellular response to amino acid starvation Source: Reactome
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Phagosome acidification Source: Reactome
Proton transmembrane transport Source: ProtInc
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Transferrin transport Source: Reactome
Cellular Location
Apical cell membrane
Involvement in disease
Cutis laxa, autosomal recessive, 2C (ARCL2C): A form of cutis laxa, a disorder characterized by an excessive congenital skin wrinkling, a large fontanelle with delayed closure, a typical facial appearance with downslanting palpebral fissures, and a general connective tissue weakness. Most ARCL2C patients exhibit severe hypotonia as well as cardiovascular involvement.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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