Mouse Anti-ATPAF1 Recombinant Antibody (E-12) (CBMAB-A4126-YC)

Provided herein is a Mouse monoclonal antibody against Human ATP Synthase Mitochondrial F1 Complex Assembly Factor 1. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ATPAF1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

E-12

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 74-230 mapping within an internal region of ATPAF1 of human origin.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

ATP Synthase Mitochondrial F1 Complex Assembly Factor 1

Introduction

ATPAF1 is an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 beta subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. Alternati

Entrez Gene ID

64756

UniProt ID

Q5TC12

Alternative Names

ATP Synthase Mitochondrial F1 Complex Assembly Factor 1; ATP11; Homolog Of Yeast ATP11; ATP11 Homolog; ATP11p;

Function

May play an essential role for the assembly of the mitochondrial F1-F0 complex.

Biological Process

Mitochondrial proton-transporting ATP synthase complex assembly Source: GO_Central

Cellular Location

Mitochondrion

References

Zhou, Z., Zhang, K., Liu, Z., Gao, X., Huang, K., Chen, C., ... & Long, Q. (2021). ATPAF1 deficiency impairs ATP synthase assembly and mitochondrial respiration. Mitochondrion, 60, 129-141.

Zhou, Z., Zhang, K., Liu, Z., Gao, X., Huang, K., Yang, Q., & Long, Q. (2020). ATPAF1 Is Essential for the Mouse Heart to Maintain Structure and Function via Controlling ATP Synthase Assembly and Mitochondrial Respiration.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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