Mouse Anti-ATXN10 Recombinant Antibody (CBFYE-0309) (CBMAB-E0591-FY)

Name: Mouse Anti-ATXN10 Recombinant Antibody (CBFYE-0309)
SKU: CBMAB-E0591-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ATXN10 Recombinant Antibody (CBFYE-0309) in WB

Ataxin-10 siRNAs or control siRNAs were transfected transiently into HUVECs. 48 h later, transfected cells were stimulated with TNF-α (10 ng/mL) for 0, 4, 8, and 24 h. Protein levels of VCAM-1, ICAM-1, and VE-cadherin were determined. ...View More

Li, Y., Zhang, Q., Li, N., Ding, L., Yi, J., Xiao, Y., ... & Huang, X. (2021). Ataxin-10 inhibits TNF-α-induced endothelial inflammation via suppressing interferon regulatory factor-1. Mediators of Inflammation, 2021.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0309

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 15-190 mapping near the N-terminus of Ataxin-10 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ataxin-10

Introduction

This gene encodes a protein that may function in neuron survival, neuron differentiation, and neuritogenesis. These roles may be carried out via activation of the mitogen-activated protein kinase cascade.

Entrez Gene ID

25814

UniProt ID

Q9UBB4

Alternative Names

E46L; SCA10; HUMEEP

Function

Necessary for the survival of cerebellar neurons. Induces neuritogenesis by activating the Ras-MAP kinase pathway. May play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.

Biological Process

Cilium assembly Source: Ensembl
Nervous system development Source: UniProtKB
Neuron projection development Source: UniProtKB

Cellular Location

Perinuclear region

Involvement in disease

Spinocerebellar ataxia 10 (SCA10): Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA10 is an autosomal dominant cerebellar ataxia (ADCA).
Defects in ATXN1 may be a cause of nephronophthisis a chronic tubulo-interstitial nephropathy that leads to anemia, polyuria, polydipsia, isosthenuria and death in uremia.

Bentley-Ford, M. R., Andersen, R. S., Croyle, M. J., Haycraft, C. J., Clearman, K. R., Foote, J. B., ... & Yoder, B. K. (2021). ATXN10 is required for embryonic heart development and maintenance of epithelial cell phenotypes in the adult kidney and pancreas. bioRxiv.

Schäfer, M., Oeing, C. U., Rohm, M., Baysal-Temel, E., Lehmann, L. H., Bauer, R., ... & Herzig, S. (2020). Corrigendum to “Ataxin-10 is part of a cachexokine cocktail triggering cardiac metabolic dysfunction in cancer cachexia”[Molecular Metabolism 5 (2)(2015) 67–78]. Molecular metabolism, 35.

Ramirez-Garcia, S. A., Cruz, F. A. L., Baltzar-Rodriguez, L. M., Sánchez-Hernández, M. A., & Elzaurdin-Mora, R. A. (2018). Genetic Perspective of the Congenital Hypothyroidism, ATXN10 a Candidate Gene. Annals Thyroid Res, 4(2), 155-155.

Tian, J., Shi, Y., Nai, S., Geng, Q., Zhang, L., Wei, G. H., ... & Li, J. (2017). Ataxin-10 is involved in Golgi membrane dynamics. Journal of Genetics and Genomics, 44(11), 549-552.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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