Rabbit Anti-AURKA/AURKB/AURKC (Phosphorylated T288, T236, T198) Monoclonal Antibody (PTM-CBPAB-0431YC) (PTM-CBPAB-0431YC)

Name: Rabbit Anti-AURKA/AURKB/AURKC (Phosphorylated T288, T236, T198) Monoclonal Antibody (PTM-CBPAB-0431YC)
SKU: PTM-CBPAB-0431YC
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-AURKA/AURKB/AURKC (Phosphorylated T288, T236, T198) Monoclonal Antibody (PTM-CBPAB-0431YC) in FC

CD532 inhibits Aurora A kinase activity and downregulates MYCN. (A-D) SKN-BE(2) cells were treated for 6 hr with the indicated compounds at 1 μM and EdU was added 1 hr prior to harvest to measure cell cycle by EdU incorporation and propidium iodide (PI) staining (A), p-H3 (B), pan-Aurora (A, B, and C isoforms) phosphorylation (C), and MYCN protein (D) by flow cytometry. ...View More

Gustafson, W. C., Meyerowitz, J. G., Nekritz, E. A., Chen, J., Benes, C., Charron, E., ... & Weiss, W. A. (2014). Drugging MYCN through an allosteric transition in Aurora kinase A. Cancer cell, 26(3), 414-427.

Rabbit Anti-AURKA/AURKB/AURKC (Phosphorylated T288, T236, T198) Monoclonal Antibody (PTM-CBPAB-0431YC) in IF

PLK1 regulates the H3T3ph-dependent phosphorylation of Aurora B/C at the inner centromeres. Double immunolabelings of SYCP3 (red) with either H3T3ph (green in A-D) or AuroraTph (green in E-H), and counterstaining of the chromatin with DAPI (blue) on squashed diakinesis (Dia.) (A,E) and metaphase I (M I) (C,G) control spermatocytes, and diakinesis (B,F) and metaphase I (D,H) BI 2536-treated spermatocytes. Yellow arrowheads indicate SYCP3 agglomerates in the nucleoplasm and cytoplasm of control diakinesis and metaphase I spermatocytes, respectively. White arrowheads in (G) indicate the centrosomes. Scale bar represents 5 μm. ...View More

Gómez, R., Viera, A., Moreno-Mármol, T., Berenguer, I., Guajardo-Grence, A., Tóth, A., ... & Suja, J. A. (2023). Kinase PLK1 regulates the disassembly of the lateral elements and the assembly of the inner centromere during the diakinesis/metaphase I transition in male mouse meiosis. Frontiers in Cell and Developmental Biology, 10, 1069946.

Rabbit Anti-AURKA/AURKB/AURKC (Phosphorylated T288, T236, T198) Monoclonal Antibody (PTM-CBPAB-0431YC) in IF

Localization of mNG-CCDC66 in RPE1 cells relative to midbody proteins. RPE1::mNG-CCDC66 cells were fixed with methanol and stained for mNeonGreen and PRC1, Cep55, Kif23 (MKLP1), or pAurora A/B/C and DAPI. Graphs show the plot profiles to assess co-localization with the indicated marker. Using ImageJ, a straight line was drawn on the midbody and intensity along the distance was plotted on Graphpad Prism. ...View More

Batman, U., Deretic, J., & Firat-Karalar, E. N. (2022). The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. PLoS Biology, 20(7), e3001708.

Rabbit Anti-AURKA/AURKB/AURKC (Phosphorylated T288, T236, T198) Monoclonal Antibody (PTM-CBPAB-0431YC) in IHC

BG+/-TMZ therapy decrease p-TOP2A, p-AURKB, p-CDC2, p-CDC20 and induce p21 in breast
tumors derived from mice. ...View More

Bobustuc, G. C., Kassam, A. B., Rovin, R. A., Jeudy, S., Smith, J. S., Isley, B., ... & Konduri, S. D. (2018). MGMT inhibition in ER positive breast cancer leads to CDC2, TOP2A, AURKB, CDC20, KIF20A, Cyclin A2, Cyclin B2, Cyclin D1, ERα and Survivin inhibition and enhances response to temozolomide. Oncotarget, 9(51), 29727.

Rabbit Anti-AURKA/AURKB/AURKC (Phosphorylated T288, T236, T198) Monoclonal Antibody (PTM-CBPAB-0431YC) in WB

Western blot analysis of phosphorylated(p)-RPS6, p-ERK1/2, p-AURKA/B/C, p-histone H3, SQSTM1/p62, LC3BI/II, γH2AX, and PARP1 (total and cleaved) in whole cell extracts from MIA PaCa-2, PANC-1, and AsPC-1 cells treated with vehicle or AD80 (0.25, 0.5, and 1 µM) for 72 h. Membranes were reprobed with the antibody to detect total protein and/or α-tubulin. ...View More

Lima, K., de Miranda, L. B. L., Del Milagro Bernabe Garnique, A., de Almeida, B. O., do Nascimento, M. C., Alcântara, G. A. S., ... & Machado-Neto, J. A. (2023). The Multikinase Inhibitor AD80 Induces Mitotic Catastrophe and Autophagy in Pancreatic Cancer Cells. Cancers, 15(15), 3866.

Datasheet

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Basic Information

Host Animal

Rabbit

Clone

PTM-CBPAB-0431YC

Application

WB, IF, FC

Immunogen

Synthetic peptide corresponding to residues surrounding Thr232 of human Aurora B.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
FC	1:50
IF(ICC)	1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aurora Kinase B/C

Introduction

Aurora kinase B/C is member of the Aurora subfamily of serine/threonine protein kinases.Aurora kinase C is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis.

Alternative Names

Aurora kinase B; Aurora kinase C

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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