Mouse Anti-B3GALT4 Recombinant Antibody (5F12B8) (CBMAB-Z0094-LY)

Name: Mouse Anti-B3GALT4 Recombinant Antibody (5F12B8)
SKU: CBMAB-Z0094-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5F12B8

Application

WB, FC, ELISA

Immunogen

Purified recombinant fragment of human B3GALT4 (AA: 191-359) expressed in E. Coli.

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:10,000
WB	1:500-1:2,000
FC	1:200-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Beta-1,3-Galactosyltransferase 4

Introduction

This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is oriented telomere to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the encoded protein is limited to ganglioseries glycolipid biosynthesis.

Entrez Gene ID

8705

UniProt ID

O96024

Alternative Names

GALT2; GALT4; BETA3GALT4

Function

Involved in GM1/GD1B/GA1 ganglioside biosynthesis.

Biological Process

Ganglioside biosynthetic process Source: BHF-UCL
Oligosaccharide biosynthetic process Source: Reactome
Protein glycosylation Source: GO_Central

Cellular Location

Golgi apparatus membrane

Topology

Cytoplasmic: 1-8 aa
Helical: 9-19 aa
Lumenal: 20-378 aa

Verma, M., & Schneider, J. S. (2019). siRNA-mediated knockdown of B3GALT4 decreases GM1 ganglioside expression and enhances vulnerability for neurodegeneration. Molecular and Cellular Neuroscience, 95, 25-30.

Zhang, T., Wang, F., Wu, J. Y., Qiu, Z. C., Wang, Y., Liu, F., ... & Hua, D. (2018). Clinical correlation of B7-H3 and B3GALT4 with the prognosis of colorectal cancer. World journal of gastroenterology, 24(31), 3538.

Madrid, A., Hogan, K. J., Papale, L. A., Clark, L. R., Asthana, S., Johnson, S. C., & Alisch, R. S. (2018). DNA hypomethylation in blood links B3GALT4 and ZADH2 to Alzheimer’s disease. Journal of Alzheimer's Disease, 66(3), 927-934.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-B3GALT4 Recombinant Antibody (CBT3791) (CAT#: V2LY-0625-LY164)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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