Mouse Anti-B4GALT4 Recombinant Antibody (CBYY-0096) (CBMAB-0096-YY)

Name: Mouse Anti-B4GALT4 Recombinant Antibody (CBYY-0096)
SKU: CBMAB-0096-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0096

Application

WB, IHC

Immunogen

Human recombinant protein fragment corresponding to amino acids 40-322 of human B4GALT4 produced in E.coli.

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500
IHC	1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Beta-1,3-Galactosyltransferase 4

Introduction

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene.

Entrez Gene ID

8702

UniProt ID

O60513

Alternative Names

Beta-1,4-Galactosyltransferase 4; UDP-Galactose:Beta-N-Acetylglucosamine Beta-1,4-Galactosyltransferase 4; UDP-Gal:BetaGlcNAc Beta 1,4- Galactosyltransferase, Polypeptide 4; UDP-Gal:Beta-GlcNAc Beta-1,4-Galactosyltransferase 4; Beta-1,4-GalTase 4; Beta4Gal-T4;

Function

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

Biological Process

Carbohydrate metabolic process Source: InterPro
Glycosylation Source: GO_Central
Keratan sulfate biosynthetic process Source: Reactome
Membrane lipid metabolic process Source: ProtInc
Protein glycosylation Source: UniProtKB-UniPathway

Cellular Location

Golgi stack membrane. Trans cisternae of Golgi stack.

Topology

Cytoplasmic: 1-12 aa
Helical: 13-38 aa
Lumenal: 39-344 aa

Dai, Z., Wang, K., & Gao, Y. (2021). The critical role of B4GALT4 in promoting microtubule spindle assembly in HCC through the regulation of PLK1 and RHAMM expression. Journal of Cellular Physiology.

Shauchuk, A., Szulc, B., Maszczak-Seneczko, D., Wiertelak, W., Skurska, E., & Olczak, M. (2020). N-glycosylation of the human β1, 4-galactosyltransferase 4 is crucial for its activity and Golgi localization. Glycoconjugate journal, 37(5), 577-588.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

B4GALT4 Matched Antibody Pair (122) (CAT#: APMAB-122LY)

Related Products

B4GALT4 Matched Antibody Pair (122) (CAT#: APMAB-122LY)

Mouse Anti-B4GALT4 Recombinant Antibody (5E2) (CAT#: CBMAB-A0689-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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