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Rabbit Anti-BABAM1 Recombinant Antibody (D7Y5K) (CBMAB-N1236-WJ)

This product is a Rabbit antibody that recognizes BABAM1. The antibody D7Y5K can be used for immunoassay techniques such as: WB, IP.
See all BABAM1 antibodies
Published Data
Rabbit Anti-BABAM1 Recombinant Antibody (D7Y5K) in WB
Western blot showing IR-induced CDC25A degradation in MCF7 cells with stably integrated CRE inducible HA-BRE expression cassette after transfection either with non-specific (NS) siRNA or two different siRNAs against MERIT40 (#1 and #2) along with CRE-expressing plasmid. Non-specific (NS) siRNA transfection was used as control. Relative abundance of CDC25A at a particular time point was measured by comparing GAPDH-normalized band intensities at that point divided by GAPDHnormalized CDC25A intensity of untreated (0 h IR) cells of corresponding treatment. ...View More
Biswas, K., Philip, S., Yadav, A., Martin, B. K., Burkett, S., Singh, V., ... & Sharan, S. K. (2018). BRE/BRCC45 regulates CDC25A stability by recruiting USP7 in response to DNA damage. Nature communications, 9(1), 537.
Rabbit Anti-BABAM1 Recombinant Antibody (D7Y5K) in IP
HEK293T cells were transfected with various combinations (above lanes) of plasmids encoding Flag-WWP2, Myc-ABRO1, Myc-BRCC3, Myc-BRE, and Myc-MERIT40. Cell lysates were immunoprecipitated with anti-c-Myc agarose and subjected to immunoblot with anti-Myc or anti-Flag antibody. ...View More
Zhang, W., Tao, S. S., Wang, T., Zhang, J., Liu, X., Li, Y. T., ... & Yin, R. H. (2021). ABRO1 stabilizes the deubiquitinase BRCC3 through inhibiting its degradation mediated by the E3 ubiquitin ligase WWP2. FEBS letters, 595(2), 169-182.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
D7Y5K
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic peptide corresponding to residues near the carboxy terminus of human MERIT40 protein.
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BRISC And BRCA1 A Complex Member 1
Introduction
BABAM1 (BRISC And BRCA1 A Complex Member 1) is a Protein Coding gene. Diseases associated with BABAM1 include Cherubism. Among its related pathways are Cell Cycle, Mitotic and Metabolism of proteins. An important paralog of this gene is ENSG00000269307.
Entrez Gene ID
Human29086
Mouse68251
Rat290631
Monkey718969
UniProt ID
HumanQ9NWV8
MouseQ3UI43
RatQ5XIJ6
MonkeyK7CLA9
Alternative Names
NBA1; HSPC142; MERIT40; C19orf62
Function
Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:24075985, PubMed:26195665).
In these 2 complexes, it is probably required to maintain the stability of BABAM2 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36 component. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665).
Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985).
Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).
Biological Process
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Chromatin organization Source: UniProtKB-KW
Double-strand break repair Source: UniProtKB
Double-strand break repair via nonhomologous end joining Source: Reactome
Positive regulation of DNA repair Source: UniProtKB
Protein deubiquitination Source: Reactome
Protein K63-linked deubiquitination Source: UniProtKB
Response to ionizing radiation Source: UniProtKB
Signal transduction involved in G2 DNA damage checkpoint Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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