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Rabbit Anti-BABAM2 Recombinant Antibody (D8Q1J) (CBMAB-CP0023-LY)

The product is antibody recognizes BABAM2. The antibody D8Q1J immunoassay techniques such as: WB,IP.
See all BABAM2 antibodies
Published Data
Rabbit Anti-BABAM2 Recombinant Antibody (D8Q1J) in WB
Western blot analysis of BABAM2 protein expression at various time points after gamma irradiation treatment. ...View More
Chung, C. Y. T., Lo, P. H. Y., & Lee, K. K. H. (2020). Babam2 Regulates Cell Cycle Progression and Pluripotency in Mouse Embryonic Stem Cells as Revealed by Induced DNA Damage. Biomedicines, 8(10), 397.
Rabbit Anti-BABAM2 Recombinant Antibody (D8Q1J) in IF
Confocal microscopic imaging of mESCs stained with anti-BABAM2 antibodies (green) demonstrating the subcellular localization of BABAM2 in mESCs with and without gamma irradiation treatment. DAPI signal (blue) indicated for the nucleus. Scale bar = 20 µm. ...View More
Chung, C. Y. T., Lo, P. H. Y., & Lee, K. K. H. (2020). Babam2 Regulates Cell Cycle Progression and Pluripotency in Mouse Embryonic Stem Cells as Revealed by Induced DNA Damage. Biomedicines, 8(10), 397.
Rabbit Anti-BABAM2 Recombinant Antibody (D8Q1J) in WB
Western-blot analysis of Babam2 gene knockdown efficiency in RAW264.7 cells. ...View More
Jin, F., Zhu, Y., Liu, M., Wang, R., Cui, Y., Wu, Y., ... & Ren, Z. (2022). Babam2 negatively regulates osteoclastogenesis by interacting with Hey1 to inhibit Nfatc1 transcription. International Journal of Biological Sciences, 18(11), 4482.
Rabbit Anti-BABAM2 Recombinant Antibody (D8Q1J) in WB
Immunoblot analysis of protein levels of the components of BRISC in BMDMs transduced with control or WWP2-expressing lentiviruses. ...View More
Zhang, W., Tao, S. S., Wang, T., Zhang, J., Liu, X., Li, Y. T., ... & Yin, R. H. (2021). ABRO1 stabilizes the deubiquitinase BRCC3 through inhibiting its degradation mediated by the E3 ubiquitin ligase WWP2. FEBS letters, 595(2), 169-182.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
D8Q1J
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Gln150 of human BRE protein.
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
BRISC And BRCA1 A Complex Member 2
Introduction
This gene encodes an anti-apoptotic, death receptor-associated protein that interacts with tumor necrosis factor-receptor-1. The encoded protein acts as an adapter in several protein complexes, including the BRCA1-A complex and the BRISC complex. The BRCA1-A complex possesses ubiquitinase activity and targets sites of double strand DNA breaks, while the BRISC complex exhibits deubiquitinase activity and is involved in mitotic spindle assembly. This gene is upregulated in several types of cancer. [provided by RefSeq, Jun 2016]
Entrez Gene ID
Human9577
Mouse107976
Rat362704
Monkey702365
UniProt ID
HumanQ9NXR7
MouseQ3UI43
RatQ6P7Q1
MonkeyK7CCA4
Alternative Names
BRISC And BRCA1 A Complex Member 2; Brain And Reproductive Organ-Expressed (TNFRSF1A Modulator); Brain And Reproductive Organ-Expressed Protein; BRCA1/BRCA2-Containing Complex; Subunit 4; BRCA1/BRCA2-Containing Complex Subunit 45; BRCA1-A Complex Subunit BRE;
Function
Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX (PubMed:17525341, PubMed:19261746, PubMed:19261749, PubMed:19261748).
In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer (PubMed:21282113, PubMed:19261748).
Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:19214193, PubMed:24075985, PubMed:25283148, PubMed:26195665).
Within the BRISC complex, acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity (PubMed:21282113).
The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665).
The BRISC complex plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985).
Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).
May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect (PubMed:15465831).
Biological Process
Apoptotic process Source: UniProtKB-KW
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cellular response to DNA damage stimulus Source: UniProtKB
Chromatin organization Source: UniProtKB-KW
Double-strand break repair Source: UniProtKB
Double-strand break repair via nonhomologous end joining Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of DNA repair Source: UniProtKB
Protein deubiquitination Source: Reactome
Protein K63-linked deubiquitination Source: UniProtKB
Response to ionizing radiation Source: UniProtKB
Signal transduction Source: ProtInc
Signal transduction involved in G2 DNA damage checkpoint Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs).
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For research use only. Not intended for any clinical use.

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