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Mouse Anti-BAG1 Recombinant Antibody (CBYY-0156) (CBMAB-0157-YY)

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Published Data
Mouse Anti-BAG1 Recombinant Antibody (CBYY-0156) in WB
Mouse Anti-BAG1 Recombinant Antibody (CBYY-0156) in WB
Western blot of nuclear extracts from hormone-deprived control (Ctr) and Bag-1L KO (KO) cells treated with vehicle (ETOH) or 10 nM DHT for 4 hr. Protein levels of Bag-1 isoforms (Bag-1L, 1S and 1, but not 1M) and AR are shown. ...View More
Cato, L., Neeb, A., Sharp, A., Buzon, V., Ficarro, S. B., Yang, L., ... & Brown, M. (2017). Development of Bag-1L as a therapeutic target in androgen receptor-dependent prostate cancer. Elife, 6, e27159.
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBYY-0156
Application
ELISA, IF, IP, WB, IHC-P
Immunogen
Amino acids 73-274 of Bag-1 of human origin.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
BCL2 Associated Athanogene 1
Introduction
The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisMouse. Multiple protein isoforMouse are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]
Entrez Gene ID
Human573
Mouse12017
Rat297994
UniProt ID
HumanQ99933
MouseQ60739
RatB0K019
Alternative Names
BCL2 Associated Athanogene 1; BAG-1; HAP; Glucocortoid Receptor-Associated Protein RAP46; BAG Family Molecular Chaperone Regulator 1; Bcl-2 Associating Athanogene-1 Protein; Receptor-Associated Protein, 46-KD;
Function
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:27474739, PubMed:9873016, PubMed:24318877).
Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity (PubMed:12724406).
Markedly increases the anti-cell death function of BCL2 induced by various stimuli (PubMed:9305631).
Biological Process
Apoptotic process Source: UniProtKB-KW
Cell surface receptor signaling pathway Source: ProtInc
Chaperone cofactor-dependent protein refolding Source: UniProtKB
Negative regulation of apoptotic process Source: ProtInc
Protein stabilization Source: GO_Central
Regulation of cellular response to heat Source: Reactome
Cellular Location
Isoform 1: Cytoplasm; Nucleus. Isoform 1 localizes predominantly to the nucleus.
Isoform 2: Cytoplasm; Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock.
Isoform 4: Cytoplasm; Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
PTM
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

Turk, M., Tatli, O., Alkan, H. F., Ozfiliz Kilbas, P., Alkurt, G., & Dinler Doganay, G. (2021). Co-Chaperone Bag-1 Plays a Role in the Autophagy-Dependent Cell Survival through Beclin 1 Interaction. Molecules, 26(4), 854.

Can, N. D., Basturk, E., Kizilboga, T., Akcay, I. M., Dingiloglu, B., Tatli, O., ... & Dinler Doganay, G. (2021). Interactome analysis of Bag-1 isoforms reveals novel interaction partners in endoplasmic reticulum-associated degradation. PloS one, 16(8), e0256640.

Luo, S., Hou, Y., Zhang, Y., Feng, L., Hunter, R. G., Yuan, P., ... & Du, J. (2021). Bag‐1 mediates glucocorticoid receptor trafficking to mitochondria after corticosterone stimulation: Potential role in regulating affective resilience. Journal of Neurochemistry, 158(2), 358-372.

Nguyen, P., Hess, K., Smulders, L., Le, D., Briseno, C., Chavez, C. M., & Nikolaidis, N. (2020). Origin and evolution of the human Bcl2-associated athanogene-1 (BAG-1). International journal of molecular sciences, 21(24), 9701.

Song, Y. K., Li, Z., Wang, Y., Qu, Y., Li, Q. S., Man, X. Y., ... & Hu, D. (2020). Inhibition of BAG‐1 induced SH‐SY5Y cell apoptosis without affecting Hsp70 expression. Journal of cellular biochemistry, 121(2), 1728-1735.

Kirschke, E., Roe-Zurz, Z., Noddings, C., & Agard, D. (2020). The interplay between Bag-1, Hsp70, and Hsp90 reveals that inhibiting Hsp70 rebinding is essential for Glucocorticoid Receptor activity. bioRxiv.

Papadakis, E. S., Reeves, T., Robson, N. H., Maishman, T., Packham, G., & Cutress, R. I. (2017). BAG-1 as a biomarker in early breast cancer prognosis: a systematic review with meta-analyses. British journal of cancer, 116(12), 1585-1594.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

BAG1 Matched Antibody Pair (123) (CAT#: APMAB-123LY)

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For research use only. Not intended for any clinical use.

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