Mouse Anti-BARHL1 Recombinant Antibody (1B7) (CBMAB-A0710-LY)

The product is antibody recognizes BARHL1. The antibody 1B7 immunoassay techniques such as: WB, ELISA.
See all BARHL1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1B7

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

BARHL1 (NP_064448, 55 a.a. ~ 154 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

BarH Like Homeobox 1

Entrez Gene ID

Human	56751
Mouse	54422
Rat	117232

UniProt ID

Human	Q9BZE3
Mouse	P63157
Rat	P63156

Biological Process

Midbrain development Source: Ensembl
Negative regulation of neuron apoptotic process Source: Ensembl
Neuron migration Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Sensory perception of sound Source: Ensembl

Cellular Location

Nucleus

References

Hou, K., Jiang, H., Karim, M., Zhong, C., Xu, Z., Liu, L., ... & Huang, X. (2019). A Critical E-box in Barhl1 3′ enhancer is essential for auditory hair cell differentiation. Cells, 8(5), 458.

Zhong, C., Chen, Z., Luo, X., Wang, C., Jiang, H., Shao, J., ... & Wang, J. (2018). Barhl1 is required for the differentiation of inner ear hair cell-like cells from mouse embryonic stem cells. The international journal of biochemistry & cell biology, 96, 79-89.

Barh, D., García-Solano, M. E., Tiwari, S., Bhattacharya, A., Jain, N., Torres-Moreno, D., ... & Perry, G. (2017). BARHL1 is downregulated in Alzheimer’s disease and may regulate cognitive functions through ESR1 and multiple pathways. Genes, 8(10), 245.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-BARHL1 Recombinant Antibody (CBYY-0197)

Mouse Anti-BARHL1 Recombinant Antibody (CBYY-0198)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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