Mouse Anti-BAZ1B Recombinant Antibody (CBWJW-167) (CBMAB-B0004-WJ)

Name: Mouse Anti-BAZ1B Recombinant Antibody (CBWJW-167)
SKU: CBMAB-B0004-WJ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-BAZ1B Recombinant Antibody (CBWJW-167) (CBMAB-B0004-WJ) in WB

Triptolide downregulates the WSTF and RPB1 signaling pathway without affecting TAB1/P38 signaling in a dose dependent manner in MV-4-11 (A) and THP-1 (B). ...View More

Kang, D., Liu, Y., Song, Y., Fang, B., Zhang, Q., & Hu, L. (2022). Triptolide shows high sensitivity and low toxicity against acute myeloid leukemia cell lines through inhibiting WSTF-RNAPII complex. Frontiers in Oncology, 12, 811850.

Mouse Anti-BAZ1B Recombinant Antibody (CBWJW-167) (CBMAB-B0004-WJ) in WB

Detection of full-length and IPA-generated truncated mRNAs by RT-PCR in normal B cells (CD5+B, BLCL) and CLL cells used in the western blot validations. ...View More

Lee, S. H., Singh, I., Tisdale, S., Abdel-Wahab, O., Leslie, C. S., & Mayr, C. (2018). Widespread intronic polyadenylation inactivates tumour suppressor genes in leukaemia. Nature, 561(7721), 127-131.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJW-167

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 1-300 mapping at the N-terminus of WSTF of human origin.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

bromodomain adjacent to zinc finger domain, 1B

Introduction

BAZ1B is a protein-coding gene encodes a member of the bromodomain protein family. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23. Diseases associated with BAZ1B include Williams-Beuren Syndrome and Chromosomal Deletion Syndrome. An important paralog of this gene is BPTF.

Entrez Gene ID

Human	9031
Mouse	22385
Rat	368002

UniProt ID

Human	Q9UIG0
Mouse	Q9Z277
Rat	G3V661

Alternative Names

WSTF; WBSCR9; WBSCR10

Function

Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication.

Biological Process

Cellular response to DNA damage stimulus Source: UniProtKB
Chromatin assembly or disassembly Source: Ensembl
Chromatin remodeling Source: BHF-UCL
Double-strand break repair Source: Reactome
Histone phosphorylation Source: UniProtKB
Positive regulation of gene expression, epigenetic Source: Reactome
Regulation of transcription by RNA polymerase II Source: BHF-UCL

Cellular Location

Nucleus. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA.

Kawaguchi, M., Nakayama, A., Aoyagi, Y., Nakamura, T., Shimizu, S., Kawamura, Y., ... & Matsuo, H. (2021). Both variants of A1CF and BAZ1B genes are associated with gout susceptibility: a replication study and meta-analysis in a Japanese population. Human cell, 34(2), 293-299.

Ohta, S., Taniguchi, T., Sato, N., Hamada, M., Taniguchi, H., & Rappsilber, J. (2019). Quantitative Proteomics of the Mitotic Chromosome Scaffold Reveals the Association of BAZ1B with Chromosomal Axes*[S]. Molecular & Cellular Proteomics, 18(2), 169-181.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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