Mouse Anti-BCAP31 Recombinant Antibody (CBYY-0272) (CBMAB-0273-YY)

Name: Mouse Anti-BCAP31 Recombinant Antibody (CBYY-0272)
SKU: CBMAB-0273-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0272

Application

ELISA, IF, IHC-P, WB, IP

Immunogen

Amino acids 157-246 mapping within a C-terminal cytoplasmic domain of BAP31 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

B Cell Receptor Associated Protein 31

Introduction

This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012]

Entrez Gene ID

10134

UniProt ID

P51572

Alternative Names

B Cell Receptor Associated Protein 31; C6-AG Tumor-Associated Antigen; DXS1357E; BAP31; B-Cell Receptor-Associated Protein 31; B-Cell Receptor Associated Protein 31; BCR-Associated Protein Bap31; BCR-Associated Protein 31;

Function

Functions as a chaperone protein (PubMed:9396746, PubMed:18287538).
Is one of the most abundant endoplasmic reticulum (ER) proteins (PubMed:9396746, PubMed:18287538).
Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD) (PubMed:9396746, PubMed:18287538).
Also serves as a cargo receptor for the export of transmembrane proteins (By similarity).
Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by stimulating the translocation of NDUFS4 and NDUFB11 from the cytosol to the mitochondria via interaction with TOMM40 (PubMed:31206022).
In response to ER stress, delocalizes from the ER-mitochondria contact sites and binds BCL2 (PubMed:31206022).
May be involved in CASP8-mediated apoptosis (PubMed:10958671).

Biological Process

Antigen processing and presentation of peptide antigen via MHC class I Source: Reactome
Apoptotic process Source: UniProtKB-KW
Calcium-mediated signaling using intracellular calcium source Source: UniProtKB
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
Negative regulation of endoplasmic reticulum calcium ion concentration Source: UniProtKB
Positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Positive regulation of ER-associated ubiquitin-dependent protein catabolic process Source: ParkinsonsUK-UCL
Positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of mitochondrial calcium ion concentration Source: UniProtKB
Positive regulation of retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
Protein localization to endoplasmic reticulum exit site Source: GO_Central
Protein targeting to mitochondrion Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Spermatogenesis Source: Ensembl
Viral process Source: UniProtKB-KW

Cellular Location

Endoplasmic reticulum membrane; Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex (PubMed:9396746). Associates with the mitochondria-associated endoplasmic reticulum membrane via interaction with TOMM40 (PubMed:31206022).

Involvement in disease

Deafness, dystonia, and cerebral hypomyelination (DDCH): An X-linked recessive mental retardation syndrome characterized by almost no psychomotor development, dysmorphic facial features, sensorineural deafness, dystonia, pyramidal signs, and hypomyelination on brain imaging.

Topology

Lumenal: 2-6 aa
Helical: 7-27 aa
Cytoplasmic: 28-43 aa
Helical: 44-64 aa
Lumenal: 65-102 aa
Helical: 103-123 aa
Cytoplasmic: 124-246 aa

PTM

Cleaved by CASP8 and other caspases.

Liang, H., Dong, J., Cheng, Z., Li, Q., Feng, D., & Ling, B. (2021). B‑cell receptor‑associated protein 31 promotes migration and invasion in ovarian cancer cells. Experimental and Therapeutic Medicine, 22(2), 1-12.

Liu, T., Yu, J., Ge, C., Zhao, F., Miao, C., Jin, W., ... & Li, H. (2021). B-cell receptor-associated protein 31 promotes metastasis via AKT/β-catenin/Snail pathway in hepatocellular carcinoma. Frontiers in molecular biosciences, 8.

Namusamba, M., Li, Z., Zhang, Q., Wang, C., Wang, T., & Wang, B. (2021). Biological roles of the B cell receptor-associated protein 31: Functional Implication in Cancer. Molecular Biology Reports, 1-14.

Abdou, A. G., Diab, A., & Marae, A. (2020). Immunohistochemical expression of BCAP 31 in chronic plaque psoriasis. Journal of Immunoassay and Immunochemistry, 41(5), 852-863.

Wang, J., Jiang, D., Li, Z., Yang, S., Zhou, J., Zhang, G., ... & Yang, K. (2020). BCAP31, a cancer/testis antigen-like protein, can act as a probe for non-small-cell lung cancer metastasis. Scientific reports, 10(1), 1-14.

Jia, C. C., Li, G., Jiang, R., Liu, X., Yuan, Q., Le, W., ... & Wang, B. (2020). B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1. Frontiers in Molecular Biosciences, 7, 64.

Nagło, O. (2019). Functional characterization of B-cell receptor associated protein 31 in cancer cells (Doctoral dissertation, lmu).

Fu, W., Sun, H., Zhao, Y., Chen, M., Yang, X., Liu, Y., & Jin, W. (2019). BCAP31 drives TNBC development by modulating ligand-independent EGFR trafficking and spontaneous EGFR phosphorylation. Theranostics, 9(22), 6468.

Jia, C. C., Du, J., Liu, X., Jiang, R., Huang, Y., Wang, T., ... & Wang, B. (2018). B-cell receptor-associated protein 31 regulates the expression of valosin-containing protein through Elf2. Cellular Physiology and Biochemistry, 51(4), 1799-1814.

Ruchusatsawat, K., Thiemsing, L., Mutirangura, A., & Wongpiyabovorn, J. (2017). BCAP 31 expression and promoter demethylation in psoriasis. Asian Pacific journal of allergy and immunology, 35(2), 86-90.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

BCAP31 Matched Antibody Pair (131) (CAT#: APMAB-131LY)

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BCAP31 Matched Antibody Pair (131) (CAT#: APMAB-131LY)

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Rat Anti-BCAP31 Recombinant Antibody (CBYY-0273) (CAT#: CBMAB-0274-YY)

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Isotype control

For research use only. Not intended for any clinical use.

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