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Mouse Anti-BCAR3 Recombinant Antibody (3G4) (CBMAB-A0726-LY)

The product is antibody recognizes BCAR3. The antibody 3G4 immunoassay techniques such as: WB, ELISA.
See all BCAR3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3G4
Antibody Isotype
IgG2a, κ
Application
WB, ELISA, IP

Basic Information

Immunogen
Amino acids 266-373 of BCAR3 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
BCAR3, NSP Family Adaptor Protein
Introduction
Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. [provided by RefSeq]
Entrez Gene ID
8412
UniProt ID
O75815
Alternative Names
KIAA0554; NSP2; SH2D3B
Function
Acts as an adapter protein downstream of several growth factor receptors to promote cell proliferation, migration, and redistribution of actin fibers (PubMed:24216110).
Specifically involved in INS/insulin signaling pathway by mediating MAPK1/ERK2-MAPK3/ERK1 activation and DNA synthesis (PubMed:24216110).
Promotes insulin-mediated membrane ruffling (By similarity).
In response to vasoconstrictor peptide EDN1, involved in the activation of RAP1 downstream of PTK2B via interaction with phosphorylated BCAR1 (PubMed:19086031).
Inhibits cell migration and invasion via regulation of TGFB-mediated matrix digestion, actin filament rearrangement, and inhibition of invadopodia activity (By similarity).
May inhibit TGFB-SMAD signaling, via facilitating BCAR1 and SMAD2 and/or SMAD3 interaction (By similarity).
Regulates EGF-induced DNA synthesis (PubMed:18722344).
Required for the maintenance of ocular lens morphology and structural integrity, potentially via regulation of focal adhesion complex signaling (By similarity).
Acts upstream of PTPRA to regulate the localization of BCAR1 and PTPRA to focal adhesions, via regulation of SRC-mediated phosphorylation of PTPRA (By similarity).
Positively regulates integrin-induced tyrosine phosphorylation of BCAR1 (By similarity).
Acts as a guanine nucleotide exchange factor (GEF) for small GTPases RALA, RAP1A and RRAS (By similarity).
However, in a contrasting study, lacks GEF activity towards RAP1 (PubMed:22081014).
Biological Process
Endothelin receptor signaling pathway Source: UniProtKB
Insulin receptor signaling pathway Source: UniProtKB
Lens morphogenesis in camera-type eye Source: Ensembl
Positive regulation of DNA replication Source: UniProtKB
Positive regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
Positive regulation of GTPase activity Source: UniProtKB
Positive regulation of MAPK cascade Source: UniProtKB
Positive regulation of peptidyl-serine phosphorylation Source: GO_Central
Response to drug Source: ProtInc
Signal transduction Source: ProtInc
Small GTPase mediated signal transduction Source: InterPro
Cellular Location
Cytoplasm; Focal adhesion. Localization to focal adhesions depends on interaction with PTPRA.
PTM
Phosphorylated on tyrosine residues.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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