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Mouse Anti-BCKDHA Recombinant Antibody (CBR060F) (CBMAB-BT005LY)

The product is antibody recognizes BCKDHA. The antibody CBR060F immunoassay techniques such as: WB.
See all BCKDHA antibodies
Published Data
Mouse Anti-BCKDHA Recombinant Antibody (CBR060F) (CBMAB-BT005LY) in Immunoblot
EGFR-WT and mutant NSCLC cells were treated with the indicated concentration of BIX for 48 h and immunoblotted with an anti-BCKDHA antibody. ...View More
Kim, J. H., Kim, J., Im, S. S., Lee, J. H., Hwang, S., Chang, E. J., ... & Son, J. (2021). BIX01294 inhibits EGFR signaling in EGFR-mutant lung adenocarcinoma cells through a BCKDHA-mediated reduction in the EGFR level. Experimental & Molecular Medicine, 53(12), 1877-1887.
Mouse Anti-BCKDHA Recombinant Antibody (CBR060F) (CBMAB-BT005LY) in WB
Western blotting analysis of BCAT2, BCKDHa, BCKDHb and Dbt in eWATs from WT and TKTAKO mice on 10-week HFD. Data are represented as mean ± SEM. Significance is determined by Student's test analysis or one-way ANOVA analysis. *P < 0.05, **P<0.01, ***P<0.001. ...View More
Tian, N., Liu, Q., Li, Y., Tong, L., Lu, Y., Zhu, Y., ... & Tong, X. (2020). Transketolase deficiency in adipose tissues protects mice from diet-induced obesity by promoting lipolysis. Diabetes, 69(7), 1355-1367.
Mouse Anti-BCKDHA Recombinant Antibody (CBR060F) (CBMAB-BT005LY) in WB
PDAC cells expressing control shRNA (shGFP) or shRNA targeting BCKDHA were assayed for cell proliferation. ...View More
Lee, J. H., Cho, Y. R., Kim, J. H., Kim, J., Nam, H. Y., Kim, S. W., & Son, J. (2019). Branched-chain amino acids sustain pancreatic cancer growth by regulating lipid metabolism. Experimental & molecular medicine, 51(11), 1-11.
Mouse Anti-BCKDHA Recombinant Antibody (CBR060F) (CBMAB-BT005LY) in WB
Protein levels of phosphorylated p-BCKDE1α and total BCKDE1αin DCs stimulated for 12 h with pRNA under indicated conditions. β-actin was used as a loading control. ...View More
Basit, F., & De Vries, I. J. M. (2019). Dendritic cells require PINK1-mediated phosphorylation of BCKDE1α to promote fatty acid oxidation for immune function. Frontiers in Immunology, 10, 2386.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBR060F
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 47-346 mapping near the N-terminus of BCKDE1A of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
branched chain keto acid dehydrogenase E1, alpha polypeptide
Introduction
The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Entrez Gene ID
Human593
Mouse12039
UniProt ID
HumanP12694
MouseP50136
Alternative Names
Branched Chain Keto Acid Dehydrogenase E1, Alpha Polypeptide; Branched-Chain Alpha-Keto Acid Dehydrogenase E1 Component Alpha Chain; 2-Oxoisovalerate Dehydrogenase (Lipoamide); BCKDH E1-Alpha; EC 1.2.4.4; BCKDE1A; Branched Chain Keto Acid Dehydrogenase E1, Alpha Polypeptide (Maple Syrup Urine Disease);
Function
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Biological Process
Branched-chain amino acid catabolic process Source: HGNC-UCL
Cellular Location
Mitochondrion matrix
Involvement in disease
Maple syrup urine disease 1A (MSUD1A): A metabolic disorder due to an enzyme defect in the catabolic pathway of the branched-chain amino acids leucine, isoleucine, and valine. Accumulation of these 3 amino acids and their corresponding keto acids leads to encephalopathy and progressive neurodegeneration. Clinical features include mental and physical retardation, feeding problems, and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine. If untreated, maple syrup urine disease can lead to seizures, coma, and death. The disease is often classified by its pattern of signs and symptoms. The most common and severe form of the disease is the classic type, which becomes apparent soon after birth. Variant forms of the disorder become apparent later in infancy or childhood and are typically milder, but they still involve developmental delay and other medical problems if not treated.
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For research use only. Not intended for any clinical use.

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