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Mouse Anti-BCKDK Recombinant Antibody (CBYY-0299) (CBMAB-0300-YY)

This product is mouse antibody that recognizes BCKDK. The antibody CBYY-0299 can be used for immunoassay techniques such as: WB, IHC, IF
See all BCKDK antibodies
Published Data
Mouse Anti-BCKDK Recombinant Antibody (CBYY-0299) (CBMAB-0300-YY) in Immunoblot
Representative immunoblot data of E1α expression in liver and heart tissues from the Control and the BCKDHA-cKO mice. e Real-time PCR analysis of BCKDHA normalized to GAPDH in different tissues as indicated. n = 3 each group, *P < 0.05. ...View More
Yu, J. Y., Cao, N., Rau, C. D., Lee, R. P., Yang, J., Flach, R. J. R., ... & Gao, C. (2023). Cell-autonomous effect of cardiomyocyte branched-chain amino acid catabolism in heart failure in mice. Acta Pharmacologica Sinica, 1-11.
Mouse Anti-BCKDK Recombinant Antibody (CBYY-0299) (CBMAB-0300-YY) in IF
Immunofluorescence analysis of BCKDK in A549 cells was performed by fluorescence microscope. ...View More
Xue, M., Xiao, J., Jiang, W., Wang, Y., Zuo, D., An, H., & Ren, L. (2023). Loss of BCAA catabolism enhances Rab1A-mTORC1 signaling activity and promotes tumor proliferation in NSCLC. Translational Oncology, 34, 101696.
Mouse Anti-BCKDK Recombinant Antibody (CBYY-0299) (CBMAB-0300-YY) in WB
The BCKDK protein level of A549 BCKDK KD and BCKDK OV stable cell lines was measured using western blotting (n = 3 for each group). ...View More
Xue, M., Xiao, J., Jiang, W., Wang, Y., Zuo, D., An, H., & Ren, L. (2023). Loss of BCAA catabolism enhances Rab1A-mTORC1 signaling activity and promotes tumor proliferation in NSCLC. Translational Oncology, 34, 101696.
Mouse Anti-BCKDK Recombinant Antibody (CBYY-0299) (CBMAB-0300-YY) in IHC
IHC staining of BCKDK in NSCLC. Representative images are shown. (200 ×, scale bar = 40 µm). ...View More
Xue, M., Xiao, J., Jiang, W., Wang, Y., Zuo, D., An, H., & Ren, L. (2023). Loss of BCAA catabolism enhances Rab1A-mTORC1 signaling activity and promotes tumor proliferation in NSCLC. Translational Oncology, 34, 101696.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-0299
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-280 mapping at the N-terminus of BCKDK of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Branched Chain Ketoacid Dehydrogenase Kinase
Introduction
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforMouse have been found for this gene. [provided by RefSeq, Dec 2012]
Entrez Gene ID
10295
UniProt ID
O14874
Alternative Names
Branched Chain Ketoacid Dehydrogenase Kinase; BCKD-Kinase; EC 2.7.11.4; BCKDHKIN; [3-Methyl-2-Oxobutanoate Dehydrogenase [Lipoamide]] Kinase, Mitochondrial; 3-Methyl-2-Oxobutanoate Dehydrogenase [Lipoamide] Kinase, Mitochondrial;
Function
Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
Biological Process
Branched-chain amino acid catabolic process Source: HGNC-UCL
Cellular amino acid catabolic process Source: UniProtKB
Isoleucine catabolic process Source: Ensembl
Leucine catabolic process Source: Ensembl
Negative regulation of cellular amino acid metabolic process Source: Reactome
Phosphorylation Source: HGNC-UCL
Protein phosphorylation Source: GO_Central
Regulation of glucose metabolic process Source: GO_Central
Spermatogenesis Source: Ensembl
Valine catabolic process Source: Ensembl
Cellular Location
Mitochondrion matrix; Mitochondrion
Involvement in disease
Branched-chain ketoacid dehydrogenase kinase deficiency (BCKDKD): This suggests that it may be possible to treat patients with mutations in BCKDK with BCAA supplementation.
Disease descriptionA metabolic disorder characterized by autism, epilepsy, intellectual disability, and reduced branched-chain amino acids.
PTM
Autophosphorylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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