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Mouse Anti-BHLHE40 Recombinant Antibody (5B1) (CBMAB-A0759-LY)

The product is antibody recognizes BHLHB2. The antibody 5B1 immunoassay techniques such as: WB, ELISA.
See all BHLHE40 antibodies
Published Data

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
5B1
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
BHLHB2 (NP_003661, 130 a.a. ~ 229 a.a) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human, Mouse
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
basic helix-loop-helix family, member e40
Introduction
This gene encodes a basic helix-loop-helix protein expressed in various tissues. Expression in the chondrocytes is responsive to the addition of Bt2cAMP. The encoded protein is believed to be involved in the control of cell differentiation. [provided by RefSeq]
Entrez Gene ID
Human8553
Mouse20893
UniProt ID
HumanO14503
MouseO35185
Alternative Names
BHLHB2; DEC1; FLJ99214; SHARP-2; STRA13; Stra14
Function
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed:12397359, PubMed:18411297).
Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop) (PubMed:14672706).
Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2 (PubMed:15193144).
Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed:15560782).
Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2 (PubMed:14672706).
Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity (PubMed:19786558).
May be involved in the regulation of chondrocyte differentiation via the cAMP pathway (PubMed:19786558).
Represses the transcription of NR0B2 and attentuates the transactivation of NR0B2 by the CLOCK-ARNTL/BMAL1 complex (PubMed:28797635).
Drives the circadian rhythm of blood pressure through transcriptional repression of ATP1B1 in the cardiovascular system (PubMed:30012868).
Biological Process
Anterior/posterior pattern specification Source: GO_Central
Circadian regulation of gene expression Source: BHF-UCL
Circadian rhythm Source: UniProtKB
Entrainment of circadian clock by photoperiod Source: BHF-UCL
Negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of circadian rhythm Source: UniProtKB
Regulation of neurogenesis Source: GO_Central
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus; Cytoplasm. Predominantly localized in the nucleus (PubMed:11278694).
PTM
Ubiquitinated; which may lead to proteasomal degradation.
Sumoylation inhibits its ubiquitination and promotes its negative regulation of the CLOCK-ARNTL/BMAL1 heterodimer transcriptional activator activity.

AJI, A., AIHEMAITI, R., ZOU, S., MAISIYITI, A., ZHANG, C., LIU, R., & SULIDAN, X. (2021). BHLHE40 modulates post-traumatic stress disorder behaviors with the involvement of the PI3K/AKT signaling pathway. Anais da Academia Brasileira de Ciências, 93.

Zhong, J. Y., Cui, X. J., Zhan, J. K., Wang, Y. J., Li, S., Lin, X., ... & Liu, Y. S. (2020). LncRNA‐ES3 inhibition by Bhlhe40 is involved in high glucose–induced calcification/senescence of vascular smooth muscle cells. Annals of the New York Academy of Sciences, 1474(1), 61-72.

Teng, Y. S., Zhao, Y. L., Li, M. S., Liu, Y. G., Cheng, P., Lv, Y. P., ... & Zhuang, Y. (2020). Upexpression of BHLHE40 in gastric epithelial cells increases CXCL12 production through interaction with p‐STAT3 in Helicobacter pylori‐associated gastritis. The FASEB Journal, 34(1), 1169-1181.

Asanoma, K., Hori, E., Yoshida, S., Yagi, H., Onoyama, I., Kodama, K., ... & Kato, K. (2019). Mutual suppression between BHLHE40/BHLHE41 and the MIR301B-MIR130B cluster is involved in epithelial-to-mesenchymal transition of endometrial cancer cells. Oncotarget, 10(45), 4640.

Huynh, J. P., Lin, C. C., Kimmey, J. M., Jarjour, N. N., Schwarzkopf, E. A., Bradstreet, T. R., ... & Stallings, C. L. (2018). Bhlhe40 is an essential repressor of IL-10 during Mycobacterium tuberculosis infection. Journal of Experimental Medicine, 215(7), 1823-1838.

Zheng, Q., Wang, C., Wang, L., Zhang, D., Liu, N., Ming, X., ... & Liu, Y. (2018). Interaction with SP1, but not binding to the E‐box motifs, is responsible for BHLHE40/DEC1‐induced transcriptional suppression of CLDN1 and cell invasion in MCF‐7 cells. Molecular carcinogenesis, 57(9), 1116-1129.

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For research use only. Not intended for any clinical use.

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