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Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY)

This product is mouse antibody that recognizes BHLHE41. The antibody CBYY-0533 can be used for immunoassay techniques such as: WB, IHC
See all BHLHE41 antibodies
Published Data
Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY) in WB
Western blotting images of DEC1, DEC2, cleaved-PARP, cleaved-caspase 3, Bcl-2, β-catenin, SOX2, c-MYC and actin treated with 50 µM cisplatin and empty vector, DEC1 or DEC2 plasmid in HeLa cells. Western blot analysis was repeated three times and similar results were obtained. ...View More
Sato, F., Bhawal, U. K., Sugiyama, N., Osaki, S., Oikawa, K., & Muragaki, Y. (2020). Potential role of DEC1 in cervical cancer cells involving overexpression and apoptosis. Clocks & sleep, 2(1), 26-38.
Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY) in IHC
Parallel expression of BHLHE40 and BHLHE41. Sixty-one primary EC specimens were used to analyze the mRNA levels of BHLHE40 (A) and BHLHE41 (B). BHLHE40/41 mRNA levels in the EC group at an early stage (stage IA) were compared with those with advanced stages (at or more than stage IB). (C) The relationship between BHLHE40 and BHLHE41 mRNA levels from the 61 specimens was analyzed using Pearson's product-moment correlation coefficient. r-values show correlation coefficients. The 61 EC samples were also analyzed for BHLHE40/41 expression levels by immunohistochemistry. Representative results are shown. A grade 1 endometrioid carcinoma (EAC) case at stage IA (D, E), another grade 2 EAC case at stage IA (F, G), a grade 3 EAC case at stage IB (H, I), and a serous carcinoma case at stage IVB (J, K). Immunohistochemical images with an anti-BHLHE40 antibody (D, F, H, J), and an anti-BHLHE41 antibody (E, G, I, K) are shown. The scale bars indicate 100 µm. The staining scores of immunohistochemical images were analyzed (L, M). The 61 cases were divided into an early stage group and an advanced stage group, as described above. (N) The relationship between BHLHE40 and BHLHE41 staining levels from the 61 specimens was analyzed using Pearson's product-moment correlation coefficient. ...View More
Asanoma, K., Hori, E., Yoshida, S., Yagi, H., Onoyama, I., Kodama, K., ... & Kato, K. (2019). Mutual suppression between BHLHE40/BHLHE41 and the MIR301B-MIR130B cluster is involved in epithelial-to-mesenchymal transition of endometrial cancer cells. Oncotarget, 10(45), 4640.
Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY) in WB
Mice were housed under light-dark conditions as described in Materials and methods. Liver, lung and muscle samples were collected from the mice and subjected to western blotting for DEC2, pAMPK, AMPK and actin levels. ...View More
Sato, F., Muragaki, Y., Kawamoto, T., Fujimoto, K., Kato, Y., & Zhang, Y. (2016). Rhythmic expression of DEC2 protein in vitro and in vivo. Biomedical Reports, 4(6), 704-710.
Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY) in WB
U2OS was cultured and subjected to a medium change. The cells were sampled at 0 and 3 h and then every 6 h until 60 h. Samples were subjected to western blotting for DEC2, pAMPK, AMPK and actin levels. ...View More
Sato, F., Muragaki, Y., Kawamoto, T., Fujimoto, K., Kato, Y., & Zhang, Y. (2016). Rhythmic expression of DEC2 protein in vitro and in vivo. Biomedical Reports, 4(6), 704-710.
Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY) in IHC
pAMPK immunoreactivity was strongly detected in DEC2 knockout mice tissues. Strong pAMPK immunoreactivity was detected in the liver and lung of DEC2 KO mice compared with WT mice. ...View More
Sato, F., Muragaki, Y., Kawamoto, T., Fujimoto, K., Kato, Y., & Zhang, Y. (2016). Rhythmic expression of DEC2 protein in vitro and in vivo. Biomedical Reports, 4(6), 704-710.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-0533
Antibody Isotype
IgG1, κ
Application
WB, IF, IP, ELISA

Basic Information

Immunogen
Amino acids 411-482 mapping at the C-terminus of DEC2 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Basic Helix-Loop-Helix Family Member E41
Introduction
This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-Bovinex binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhytHuman and cell differentiation. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, Feb 2014]
Entrez Gene ID
79365
UniProt ID
Q9C0J9
Alternative Names
Basic Helix-Loop-Helix Family Member E41; Enhancer-Of-Split And Hairy-Related Protein 1; Basic Helix-Loop-Helix Domain Containing, Class B, 3; Differentially Expressed In Chondrocytes Protein 2; BHLHB3; SHARP1; HDEC2; DEC2;
Function
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed:11278948, PubMed:14672706, PubMed:15193144, PubMed:15560782, PubMed:18411297, PubMed:19786558, PubMed:25083013).
Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed:25083013).
Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity).
Biological Process
Anterior/posterior pattern specification Source: GO_Central
Circadian regulation of gene expression Source: GO_Central
Negative regulation of myotube differentiation Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by competitive promoter binding Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of neurogenesis Source: GO_Central
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

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