Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY)

Name: Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533)
SKU: CBMAB-0536-YY
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Published Data

Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY) in WB

Western blotting images of DEC1, DEC2, cleaved-PARP, cleaved-caspase 3, Bcl-2, β-catenin, SOX2, c-MYC and actin treated with 50 µM cisplatin and empty vector, DEC1 or DEC2 plasmid in HeLa cells. Western blot analysis was repeated three times and similar results were obtained. ...View More

Sato, F., Bhawal, U. K., Sugiyama, N., Osaki, S., Oikawa, K., & Muragaki, Y. (2020). Potential role of DEC1 in cervical cancer cells involving overexpression and apoptosis. Clocks & sleep, 2(1), 26-38.

Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY) in IHC

Parallel expression of BHLHE40 and BHLHE41. Sixty-one primary EC specimens were used to analyze the mRNA levels of BHLHE40 (A) and BHLHE41 (B). BHLHE40/41 mRNA levels in the EC group at an early stage (stage IA) were compared with those with advanced stages (at or more than stage IB). (C) The relationship between BHLHE40 and BHLHE41 mRNA levels from the 61 specimens was analyzed using Pearson's product-moment correlation coefficient. r-values show correlation coefficients. The 61 EC samples were also analyzed for BHLHE40/41 expression levels by immunohistochemistry. Representative results are shown. A grade 1 endometrioid carcinoma (EAC) case at stage IA (D, E), another grade 2 EAC case at stage IA (F, G), a grade 3 EAC case at stage IB (H, I), and a serous carcinoma case at stage IVB (J, K). Immunohistochemical images with an anti-BHLHE40 antibody (D, F, H, J), and an anti-BHLHE41 antibody (E, G, I, K) are shown. The scale bars indicate 100 µm. The staining scores of immunohistochemical images were analyzed (L, M). The 61 cases were divided into an early stage group and an advanced stage group, as described above. (N) The relationship between BHLHE40 and BHLHE41 staining levels from the 61 specimens was analyzed using Pearson's product-moment correlation coefficient. ...View More

Asanoma, K., Hori, E., Yoshida, S., Yagi, H., Onoyama, I., Kodama, K., ... & Kato, K. (2019). Mutual suppression between BHLHE40/BHLHE41 and the MIR301B-MIR130B cluster is involved in epithelial-to-mesenchymal transition of endometrial cancer cells. Oncotarget, 10(45), 4640.

Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY) in WB

Mice were housed under light-dark conditions as described in Materials and methods. Liver, lung and muscle samples were collected from the mice and subjected to western blotting for DEC2, pAMPK, AMPK and actin levels. ...View More

Sato, F., Muragaki, Y., Kawamoto, T., Fujimoto, K., Kato, Y., & Zhang, Y. (2016). Rhythmic expression of DEC2 protein in vitro and in vivo. Biomedical Reports, 4(6), 704-710.

Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY) in WB

U2OS was cultured and subjected to a medium change. The cells were sampled at 0 and 3 h and then every 6 h until 60 h. Samples were subjected to western blotting for DEC2, pAMPK, AMPK and actin levels. ...View More

Sato, F., Muragaki, Y., Kawamoto, T., Fujimoto, K., Kato, Y., & Zhang, Y. (2016). Rhythmic expression of DEC2 protein in vitro and in vivo. Biomedical Reports, 4(6), 704-710.

Mouse Anti-BHLHE41 Recombinant Antibody (CBYY-0533) (CBMAB-0536-YY) in IHC

pAMPK immunoreactivity was strongly detected in DEC2 knockout mice tissues. Strong pAMPK immunoreactivity was detected in the liver and lung of DEC2 KO mice compared with WT mice. ...View More

Sato, F., Muragaki, Y., Kawamoto, T., Fujimoto, K., Kato, Y., & Zhang, Y. (2016). Rhythmic expression of DEC2 protein in vitro and in vivo. Biomedical Reports, 4(6), 704-710.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0533

Application

WB, IF, IP, ELISA

Immunogen

Amino acids 411-482 mapping at the C-terminus of DEC2 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Basic Helix-Loop-Helix Family Member E41

Introduction

This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-Bovinex binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhytHuman and cell differentiation. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, Feb 2014]

Entrez Gene ID

79365

UniProt ID

Q9C0J9

Alternative Names

Basic Helix-Loop-Helix Family Member E41; Enhancer-Of-Split And Hairy-Related Protein 1; Basic Helix-Loop-Helix Domain Containing, Class B, 3; Differentially Expressed In Chondrocytes Protein 2; BHLHB3; SHARP1; HDEC2; DEC2;

Function

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed:11278948, PubMed:14672706, PubMed:15193144, PubMed:15560782, PubMed:18411297, PubMed:19786558, PubMed:25083013).
Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed:25083013).
Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity).

Biological Process

Anterior/posterior pattern specification Source: GO_Central
Circadian regulation of gene expression Source: GO_Central
Negative regulation of myotube differentiation Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by competitive promoter binding Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of neurogenesis Source: GO_Central
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

Mamoor, S. (2020). The transcription factor and basic helix-loop-helix family member e41 BHLHE41 is differentially expressed and transcriptionally induced in models of coronavirus infection.

Zhang, D., Zheng, Q., Wang, C., Zhao, N., Liu, Y., & Wang, E. (2020). BHLHE41 suppresses MCF‐7 cell invasion via MAPK/JNK pathway. Journal of cellular and molecular medicine, 24(7), 4001-4010.

Wang, C., Zhao, N., Zheng, Q., Zhang, D., & Liu, Y. (2019). BHLHE41 promotes U87 and U251 cell proliferation via ERK/cyclinD1 signaling pathway. Cancer management and research, 11, 7657.

Sun, Y., Zhang, H., Wang, L., Li, J., Jin, H., Wang, Z., ... & Liu, X. (2019). Loss of the basic helix-loop-helix transcription factor Bhlhe41 induces cell death and impairs neurite outgrowth in Neuro2a cells. Molecular and cellular biochemistry, 450(1-2), 167-174.

Shen, Z., Zhu, L., Zhang, C., Cui, X., & Lu, J. (2019). Overexpression of BHLHE41, correlated with DNA hypomethylation in 3'UTR region, promotes the growth of human clear cell renal cell carcinoma. Oncology reports, 41(4), 2137-2147.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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