Mouse Anti-BHMT Recombinant Antibody (CBYY-0548) (CBMAB-0551-YY)

Name: Mouse Anti-BHMT Recombinant Antibody (CBYY-0548)
SKU: CBMAB-0551-YY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-BHMT Recombinant Antibody (CBYY-0548) (CBMAB-0551-YY) in Immunoblot

HNF4α depletion reduces the protein levels of key SAA metabolic enzymes. HepG2 cells were transfected with control siRNA (siNeg) or a siRNA targeting HNF4α (siHNF4α) for 48 h (representative immunoblots are shown from at least three independent experiments). ...View More

Xu, Q., Li, Y., Gao, X., Kang, K., Williams, J. G., Tong, L., ... & Li, X. (2020). HNF4α regulates sulfur amino acid metabolism and confers sensitivity to methionine restriction in liver cancer. Nature communications, 11(1), 3978.

Mouse Anti-BHMT Recombinant Antibody (CBYY-0548) (CBMAB-0551-YY) in Immunoblot

Protein expression of key SAA metabolic enzymes in 3 selected epithelial and 2 mesenchymal liver cancer cell lines (representative immunoblots are shown from at least three independent experiments). ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0548

Application

ELISA, IF, IP, WB, IHC-P

Immunogen

Amino acids 361-406 mapping at the C-terminus of BHMT of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Betaine--Homocysteine S-Methyltransferase

Introduction

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.

Entrez Gene ID

635

UniProt ID

Q93088

Alternative Names

Betaine--Homocysteine S-Methyltransferase; EC 2.1.1.5; Epididymis Secretory Sperm Binding Protein Li 61p; Betaine--Homocysteine S-Methyltransferase 1; Betaine Homocysteine Methyltransferase; HEL-S-61p; EC 2.1.1; BHMT1;

Function

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Biological Process

Amino-acid betaine catabolic process Source: UniProtKB-UniPathway
Amino-acid betaine metabolic process Source: BHF-UCL
Choline catabolic process Source: Reactome
L-methionine salvage Source: BHF-UCL
Protein methylation Source: UniProtKB
Regulation of homocysteine metabolic process Source: UniProtKB
Sulfur amino acid metabolic process Source: Reactome

Cellular Location

Cytoplasm

Sternbach, S., West, N., Singhal, N. K., Clements, R., Basu, S., Tripathi, A., ... & McDonough, J. (2021). The BHMT-betaine methylation pathway epigenetically modulates oligodendrocyte maturation. PloS one, 16(5), e0250486.

Na, J. D., Choi, Y. J., Jun, D. S., & Kim, Y. C. (2019). Alleviation of paraquat-induced oxidative lung injury by betaine via regulation of sulfur-containing amino acid metabolism despite the lack of betaine-homocysteine methyltransferase (BHMT) in the lung. Food & function, 10(2), 1225-1234.

Maclean, K. N., Jiang, H., Phinney, W. N., Keating, A. K., Hurt, K. J., & Stabler, S. P. (2019). Taurine alleviates repression of betaine‐homocysteine S‐methyltransferase and significantly improves the efficacy of long‐term betaine treatment in a mouse model of cystathionine β‐synthase–deficient homocystinuria. The FASEB Journal, 33(5), 6339-6353.

Saande, C. J., Pritchard, S. K., Worrall, D. M., Snavely, S. E., Nass, C. A., Neuman, J. C., ... & Schalinske, K. L. (2019). Dietary egg protein prevents hyperhomocysteinemia via upregulation of hepatic betaine-homocysteine s-methyltransferase activity in folate-restricted rats. The Journal of nutrition, 149(8), 1369-1376.

Partearroyo, T., Murillo‐Cuesta, S., Vallecillo, N., Bermúdez‐Muñoz, J. M., Rodríguez‐de LaRosa, L., Mandruzzato, G., ... & Varela‐Nieto, I. (2019). Betaine‐homocysteine S‐methyltransferase deficiency causes increased susceptibility to noise‐induced hearing loss associated with plasma hyperhomocysteinemia. The FASEB Journal, 33(5), 5942-5956.

Wu, L., Zhou, X., Li, T., He, J., Huang, L., Ouyang, Z., ... & He, Q. (2018). Improved Sp1 and betaine homocysteine-S-methyltransferase expression and homocysteine clearance are involved in the effects of zinc on oxidative stress in high-fat-diet-pretreated mice. Biological trace element research, 184(2), 436-441.

Garrido, F., Pacheco, M., Vargas-Martínez, R., Velasco-García, R., Jorge, I., Serrano, H., ... & Pajares, M. Á. (2018). Identification of hepatic protein-protein interaction targets for betaine homocysteine S-methyltransferase. PloS one, 13(6), e0199472.

Prieur, E. A., Pjetri, E., Zeisel, S. H., & Jadavji, N. M. (2017). Reduced brain volume and impaired memory in betaine homocysteine S-methyltransferase knockout mice. Applied Physiology, Nutrition, and Metabolism, 42(11), 1228-1231.

Pérez-Miguelsanz, J., Vallecillo, N., Garrido, F., Reytor, E., Pérez-Sala, D., & Pajares, M. A. (2017). Betaine homocysteine S-methyltransferase emerges as a new player of the nuclear methionine cycle. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1864(7), 1165-1182.

Mostashari-Mohases, M., Sadeghi, A. A., Ahmadi, J., & Esmaeilkhanian, S. (2017). Effect of Betaine Supplementation on Performance Parameters, Betaine-homocysteine S-methyltransferase Gene Expression in Broiler Chickens Consume Drinking Water with Different Total Dissolved Solids. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(4).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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