Mouse Anti-BIK Recombinant Antibody (CBYY-0585) (CBMAB-0588-YY)

Name: Mouse Anti-BIK Recombinant Antibody (CBYY-0585)
SKU: CBMAB-0588-YY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-BIK Recombinant Antibody (CBYY-0585) (CBMAB-0588-YY) in IP

Co-immunoprecipitation analysis for BID, BIK, and PUMA binding proteins after 24 h treatment. Input levels of the immunoprecipitated proteins are shown below. ...View More

Zhu, R., Li, L., Nguyen, B., Seo, J., Wu, M., Seale, T., ... & Small, D. (2021). FLT3 tyrosine kinase inhibitors synergize with BCL-2 inhibition to eliminate FLT3/ITD acute leukemia cells through BIM activation. Signal transduction and targeted therapy, 6(1), 186.

Mouse Anti-BIK Recombinant Antibody (CBYY-0585) (CBMAB-0588-YY) in WB

AML cell lines were treated with 20 or 80 nM single-agent alvocidib for 24 hours, and BCL-2 family proteins quantified by western blot of duplicate biological extracts. ...View More

Bogenberger, J., Whatcott, C., Hansen, N., Delman, D., Shi, C. X., Kim, W., ... & Tibes, R. (2017). Combined venetoclax and alvocidib in acute myeloid leukemia. Oncotarget, 8(63), 107206.

Mouse Anti-BIK Recombinant Antibody (CBYY-0585) (CBMAB-0588-YY) in WB

Western blot analysis of the wild-type (WT), OctaKO clone (cln) B, and two Bcl-2 allKO clones for Bcl-2 family proteins. The asterisk indicates a nonspecific protein. ...View More

O'Neill, K. L., Huang, K., Zhang, J., Chen, Y., & Luo, X. (2016). Inactivation of prosurvival Bcl-2 proteins activates Bax/Bak through the outer mitochondrial membrane. Genes & development, 30(8), 973-988.

Mouse Anti-BIK Recombinant Antibody (CBYY-0585) (CBMAB-0588-YY) in WB

Western blot of the indicated cell lines. An asterisk indicates a nonspecific protein. ...View More

Mouse Anti-BIK Recombinant Antibody (CBYY-0585) (CBMAB-0588-YY) in WB

Western blot of the cell lysates of the indicated cell lines with the indicated antibodies. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0585

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 1-160 representing full length NBK (Natural Born Killer) of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BCL2-interacting killer (apoptosis-inducing)

Introduction

The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.

Entrez Gene ID

638

UniProt ID

Q13323

Alternative Names

BCL2 Interacting Killer; BCL2-Interacting Killer (Apoptosis-Inducing); Apoptosis Inducer NBK; Natural Born Killer; BIP1; BP4; NBK; Bcl-2-Interacting Killer; Apoptosis-Inducing NBK;

Function

Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.

Biological Process

Apoptotic mitochondrial changes Source: GO_Central
Apoptotic process Source: UniProtKB
Male gonad development Source: GO_Central
Positive regulation of protein-containing complex assembly Source: BHF-UCL
Positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
Regulation of apoptotic process Source: InterPro

Cellular Location

Mitochondrion membrane; Endomembrane system. Around the nuclear envelope, and in cytoplasmic membranes.

Topology

Helical: 136-156 aa

PTM

Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage is a necessary step prior its degradation by the proteosome-dependent mechanism.

López-Muñoz, E., Corres-Molina, M., & García-Hernández, N. (2020). Correlation of the protein expression of GRP78 and BIK/NBK with prognostic markers in patients with breast cancer and neoadjuvant chemotherapy. Journal of Obstetrics and Gynaecology, 40(3), 419-426.

Pandya, V., Githaka, J. M., Patel, N., Veldhoen, R., Hugh, J., Damaraju, S., ... & Goping, S. (2020). BIK drives an aggressive breast cancer phenotype through sublethal apoptosis and predicts poor prognosis of ER-positive breast cancer. Cell death & disease, 11(6), 1-19.

Chen, X., Li, C., Li, J., Sheng, L., & Liu, X. (2019). Upregulation of miR-1306-5p decreases cerebral ischemia/reperfusion injury in vitro by targeting BIK. Bioscience, biotechnology, and biochemistry, 83(12), 2230-2237.

Pandya, V. K. (2019). Investigation of BCL-2 interacting killer (BIK) as a breast cancer biomarker and its role in failed apoptosis.

Miguel, V. F., REG R, K. A., Javier, T. L., & Juan, R. C. (2018). Accurate Identification of BIK Binding Sites at the MDA-MB-231 Cell Genome by Human Tiling Arrays. Cancer Biol Ther Oncol, 2(1), 2.

Mebratu, Y. A., & Tesfaigzi, Y. (2018). Does the BCL-2 family member BIK control lung carcinogenesis?. Molecular & cellular oncology, 5(4), e1435182.

Mebratu, Y. A., & Tesfaigzi, Y. (2018). Bcl-2 Interacting Killer (BIK) Augments Influenza A Virus Replication and Severity of Flu. In B62. LUNG CYTOPROTECTION AND IMMUNITY DURING INFECTIONS (pp. A3845-A3845). American Thoracic Society.

Lin, M. L., & Chen, S. S. (2017). Activation of Casein Kinase II by Gallic Acid Induces BIK–BAX/BAK-Mediated ER Ca++-ROS-Dependent Apoptosis of Human Oral Cancer Cells. Frontiers in physiology, 8, 761.

Borst, A., Haferkamp, S., Grimm, J., Rösch, M., Zhu, G., Guo, S., ... & Houben, R. (2017). BIK is involved in BRAF/MEK inhibitor induced apoptosis in melanoma cell lines. Cancer letters, 404, 70-78.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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