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Mouse Anti-BLZF1 Recombinant Antibody (2E10) (CBMAB-A0804-LY)

The product is antibody recognizes BLZF1. The antibody 2E10 immunoassay techniques such as: WB, ELISA.
See all BLZF1 antibodies
Published Data
Fig.1 Immunoprecipitations (IPs) were performed from 20 p-g Golgi membranes with the following antibodies: sheep anti-GRASP55, mouse anti-GRASP65, sheep anti-GM130, and rabbit anti-golgin-45.
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Fig.2 Golgin-45 behaves like a Golgi matrix protein upon BFA treatment.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
2E10
Antibody Isotype
IgG2a, κ
Application
WB, E

Basic Information

Immunogen
BLZF1 (NP_003657, 301 a.a. ~ 400 a.a) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Basic Leucine Zipper Nuclear Factor 1
Entrez Gene ID
8548
UniProt ID
Q9H2G9
Alternative Names
GOLGIN-45; JEM-1; JEM-1s; JEM1; MGC22497
Function
Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
Biological Process
Golgi organization Source: MGI
Golgi to plasma membrane protein transport Source: MGI
Cellular Location
Golgi apparatus membrane
Isoform 1: Nucleus. Detected in the nucleus upon heterologous expression. Not detected in the cytoplasm.
Isoform 2: Cytoplasm. Not detected in the nucleus.
PTM
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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