Mouse Anti-BNIP3L Recombinant Antibody (3G2) (CBMAB-A0845-LY)

Name: Mouse Anti-BNIP3L Recombinant Antibody (3G2)
SKU: CBMAB-A0845-LY
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Published Data

CBMAB-A0845-LY in WB

Western blot analyses of Mieap-induced cell death in gastric cancer cells.
To clarify the association between Mieap and proapoptotic/proautophagic molecules, western blotting was performed using (A) MKN1, (B) MKN45, and (C) TMK1 cells. After 48-72 h of adenovirus treatment, cleaved caspase-3/7/8/9 and cleaved PARP were examined. Cleaved caspases are indicated with arrows. All the experimental procedures were previously indicated. No infection is denoted by (−). Ad-EGFP, Ad-BNIP3, and Ad-Δ817 of Mieap were considered negative controls. ...View More

Sano, H., Futamura, M., Gaowa, S., Kamino, H., Nakamura, Y., Yamaguchi, K., ... & Yoshida, K. (2020). p53/Mieap-regulated mitochondrial quality control plays an important role as a tumor suppressor in gastric and esophageal cancers. Biochemical and Biophysical Research Communications, 529(3), 582-589.

CBMAB-A0845-LY in IP

The interaction between endogenous Mieap and NIX/BNIP3. The control A549 cells were γ-irradiated, and 2 days later, the cell lysates were purified from the mitochondrial fraction. ...View More

Nakamura, Y., Kitamura, N., Shinogi, D., Yoshida, M., Goda, O., Murai, R., ... & Arakawa, H. (2012). BNIP3 and NIX mediate Mieap-induced accumulation of lysosomal proteins within mitochondria. PloS one, 7(1), e30767.

CBMAB-A0845-LY in IP

The mutants lacking coiled-coil regions of Mieap α and Mieap β proteins fail to bind NIX and BNIP3. HCT116 cells were transfected with plasmids expressing FLAG-NIX and FLAG-BNIP3 and infected with adenoviral vectors expressing the various Mieap α and Mieap β mutants. Cell lysates were isolated 2 days after transfection and infection and subjected to IP experiments. FLAG-NIX and FLAG-BNIP3 or the Mieap α and Mieap β mutants were precipitated using anti-FLAG or anti-Mieap antibodies, respectively. The precipitates were subjected to western blot analysis using anti-Mieap or anti-FLAG antibodies. ...View More

CBMAB-A0845-LY in IF

Immunofluorescence (IF) experiments. HCT116 cells were infected with Ad-Mieap β and Ad-DsRed-mito at an MOI of 5 and transfected with plasmids expressing FLAG-BNIP3 or FLAG-NIX. After 2 days, the cells were subjected to IF analysis using the anti-Mieap antibody (red) and the anti-FLAG antibody (green). The mitochondria were detected using DsRed-mito (red). The arrowheads indicate the representative data for BNIP3, NIX, DsRedmito, and Mieap. Scale bar = 10 µm. ...View More

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3G2

Application

WB, E, IF

Immunogen

BNIP3L (NP_004322, 43 a.a. ~ 130 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

BCL2 interacting protein 3 like

Introduction

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. The protein encoded by this gene is a functional homolog of BNIP3, a proapoptotic protein. This protein may function simultaneously with BNIP3 and may play a role in tumor suppression. [provided by RefSeq]

Entrez Gene ID

665

UniProt ID

O60238

Alternative Names

BNIP3a; NIX

Function

Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor.

Biological Process

Cellular response to hypoxia Source: MGI
Defense response to virus Source: UniProtKB
Mitochondrial outer membrane permeabilization Source: GO_Central
Mitochondrial protein catabolic process Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cell death Source: MGI
Negative regulation of mitochondrial membrane potential Source: Ensembl
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of macroautophagy Source: MGI
Regulation of apoptotic process Source: Reactome
Regulation of autophagy of mitochondrion Source: Ensembl
Regulation of programmed cell death Source: GO_Central
Regulation of protein targeting to mitochondrion Source: Ensembl
Viral process Source: UniProtKB-KW

Cellular Location

Mitochondrion outer membrane; Endoplasmic reticulum; Nucleus envelope; Membrane. Colocalizes with SPATA18 at the mitochondrion outer membrane.

Topology

Helical: 188-208 aa

PTM

Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.

Li, R., Chen, G., Dang, Y., He, R., Liu, A., Ma, J., & Ling, Z. (2021). Expression and Clinical Significance of BCL2 Interacting Protein 3 Like in Multiple Myeloma. Technology in cancer research & treatment, 20, 15330338211024551.

Kim, Y. J., Choo, O. S., Lee, J. S., Jang, J. H., Woo, H. G., & Choung, Y. H. (2021). BCL2 Interacting Protein 3-like/NIX-mediated Mitophagy Plays an Important Role in the Process of Age-related Hearing Loss. Neuroscience, 455, 39-51.

Marinković, M., Šprung, M., & Novak, I. (2021). Dimerization of mitophagy receptor BNIP3L/NIX is essential for recruitment of autophagic machinery. Autophagy, 17(5), 1232-1243.

Wu, X., Zheng, Y., Liu, M., Li, Y., Ma, S., Tang, W., ... & Zhang, X. (2021). BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains. Autophagy, 17(8), 1934-1946.

Yazdankhah, M., Ghosh, S., Shang, P., Stepicheva, N., Hose, S., Liu, H., ... & Sinha, D. (2021). BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes. Autophagy, 1-20.

Ouyang, S., Chen, W., Zeng, G., Lei, C., Tian, G., Zhu, M., ... & Yang, M. (2020). MicroRNA-183-3p up-regulated by vagus nerve stimulation mitigates chronic systolic heart failure via the reduction of BNIP3L-mediated autophagy. Gene, 726, 144136.

da Silva Rosa, S. C., Martens, M. D., Field, J. T., Nguyen, L., Kereliuk, S. M., Hai, Y., ... & Gordon, J. W. (2020). BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation. Autophagy, 1-16.

Lampert, M. A., Orogo, A. M., Najor, R. H., Hammerling, B. C., Leon, L. J., Wang, B. J., ... & Gustafsson, Å. B. (2019). BNIP3L/NIX and FUNDC1-mediated mitophagy is required for mitochondrial network remodeling during cardiac progenitor cell differentiation. Autophagy, 15(7), 1182-1198.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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