Sign in or Register   Sign in or Register
  |  

Mouse Anti-BNIP3L Recombinant Antibody (3G2) (CBMAB-A0845-LY)

The product is antibody recognizes BNIP3L. The antibody 3G2 immunoassay techniques such as: WB, ELISA.
See all BNIP3L antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3G2
Antibody Isotype
IgG1, κ
Application
WB, E, IF

Basic Information

Immunogen
BNIP3L (NP_004322, 43 a.a. ~ 130 a.a) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
BCL2 interacting protein 3 like
Introduction
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. The protein encoded by this gene is a functional homolog of BNIP3, a proapoptotic protein. This protein may function simultaneously with BNIP3 and may play a role in tumor suppression. [provided by RefSeq]
Entrez Gene ID
665
UniProt ID
O60238
Alternative Names
BNIP3a; NIX
Function
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor.
Biological Process
Cellular response to hypoxia Source: MGI
Defense response to virus Source: UniProtKB
Mitochondrial outer membrane permeabilization Source: GO_Central
Mitochondrial protein catabolic process Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cell death Source: MGI
Negative regulation of mitochondrial membrane potential Source: Ensembl
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of macroautophagy Source: MGI
Regulation of apoptotic process Source: Reactome
Regulation of autophagy of mitochondrion Source: Ensembl
Regulation of programmed cell death Source: GO_Central
Regulation of protein targeting to mitochondrion Source: Ensembl
Viral process Source: UniProtKB-KW
Cellular Location
Mitochondrion outer membrane; Endoplasmic reticulum; Nucleus envelope; Membrane. Colocalizes with SPATA18 at the mitochondrion outer membrane.
Topology
Helical: 188-208 aa
PTM
Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.

Li, R., Chen, G., Dang, Y., He, R., Liu, A., Ma, J., & Ling, Z. (2021). Expression and Clinical Significance of BCL2 Interacting Protein 3 Like in Multiple Myeloma. Technology in cancer research & treatment, 20, 15330338211024551.

Kim, Y. J., Choo, O. S., Lee, J. S., Jang, J. H., Woo, H. G., & Choung, Y. H. (2021). BCL2 Interacting Protein 3-like/NIX-mediated Mitophagy Plays an Important Role in the Process of Age-related Hearing Loss. Neuroscience, 455, 39-51.

Marinković, M., Šprung, M., & Novak, I. (2021). Dimerization of mitophagy receptor BNIP3L/NIX is essential for recruitment of autophagic machinery. Autophagy, 17(5), 1232-1243.

Wu, X., Zheng, Y., Liu, M., Li, Y., Ma, S., Tang, W., ... & Zhang, X. (2021). BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains. Autophagy, 17(8), 1934-1946.

Yazdankhah, M., Ghosh, S., Shang, P., Stepicheva, N., Hose, S., Liu, H., ... & Sinha, D. (2021). BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes. Autophagy, 1-20.

Ouyang, S., Chen, W., Zeng, G., Lei, C., Tian, G., Zhu, M., ... & Yang, M. (2020). MicroRNA-183-3p up-regulated by vagus nerve stimulation mitigates chronic systolic heart failure via the reduction of BNIP3L-mediated autophagy. Gene, 726, 144136.

da Silva Rosa, S. C., Martens, M. D., Field, J. T., Nguyen, L., Kereliuk, S. M., Hai, Y., ... & Gordon, J. W. (2020). BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation. Autophagy, 1-16.

Lampert, M. A., Orogo, A. M., Najor, R. H., Hammerling, B. C., Leon, L. J., Wang, B. J., ... & Gustafsson, Å. B. (2019). BNIP3L/NIX and FUNDC1-mediated mitophagy is required for mitochondrial network remodeling during cardiac progenitor cell differentiation. Autophagy, 15(7), 1182-1198.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-BNIP3L Recombinant Antibody (3G2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare