Mouse Anti-BOLA1 Recombinant Antibody (2H3) (CBMAB-A0847-LY)

Name: Mouse Anti-BOLA1 Recombinant Antibody (2H3)
SKU: CBMAB-A0847-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2H3

Antibody Isotype

IgG1, κ

Application

WB, E

Basic Information

Immunogen

BOLA1 (NP_057158, 38 a.a. ~ 137 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

bolA homolog 1 (E. coli)

Entrez Gene ID

51027

UniProt ID

Q9Y3E2

Alternative Names

CGI-143; MGC75015; RP11-196G18.18

Function

Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates (Fe-S) cluster insertion into a subset of mitochondrial proteins (By similarity).
Probably acts together with the monothiol glutaredoxin GLRX5 (PubMed:27532772).
May protect cells against oxidative stress (PubMed:22746225).

Cellular Location

Mitochondrion

More Infomation

References

Zhu, M., & Xiao, S. (2021). Expression profiles and prognostic values of BolA family members in ovarian cancer. Journal of Ovarian Research, 14(1), 1-12.

Nasta, V., Giachetti, A., Ciofi-Baffoni, S., & Banci, L. (2017). Structural insights into the molecular function of human [2Fe-2S] BOLA1-GRX5 and [2Fe-2S] BOLA3-GRX5 complexes. Biochimica et Biophysica Acta (BBA)-General Subjects, 1861(8), 2119-2131.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

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