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  • Mouse Anti-BOP1 Recombinant Antibody (XB0102)

Mouse Anti-BOP1 Recombinant Antibody (XB0102) (CBMAB-XB0102-YC)

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Published Data
CBMAB-XB0102-YC-1
CBMAB-XB0102-YC in IF
Flag-SOD1 is colocalized with BOP1 and WDR12. Sod1Flox/Flox KP cells stably expressing Flag-SOD1 were treated with 4OHT and doxycycline to induce knockout of endogenous Sod1 and expression of ectopic Flag-SOD1, respectively. IF analysis was performed with antibodies against Flag (green) and BOP1 or WDR12 (red). Dashed lines indicate the nucleus. Scale bar represents 10 μm. Data are represented by three independent experiments. ...View More
Wang, X., Zhang, H., Sapio, R., Yang, J., Wong, J., Zhang, X., ... & Steven Zheng, X. F. (2021). SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer. Nature communications, 12(1), 2259.
CBMAB-XB0102-YC-2
CBMAB-XB0102-YC in IF
SOD1 knockdown causes delocalization of BOP1 from pre-rRNA. Mouse NSCLC cells were transfected with mouse SOD1 siRNA (siSOD1) or a control siRNA (siNC). BOP1 and pre-rRNA were stained by IF and RNA-FISH, respectively. Scale bar represents 10 μm. Data are represented by three independent experiments. ...View More
Wang, X., Zhang, H., Sapio, R., Yang, J., Wong, J., Zhang, X., ... & Steven Zheng, X. F. (2021). SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer. Nature communications, 12(1), 2259.
CBMAB-XB0102-YC-3
CBMAB-XB0102-YC in WB
Immunoblotting for nucleolar protein BOP1 and RRP1 in ASA-WT and ASA-KO cells cultured in 10% or 1% FBS containing media with or without acetate for 4 hours. αTubulin was used as a loading control. ...View More
Houston, R., Sekine, S., Calderon, M. J., Seifuddin, F., Wang, G., Kawagishi, H., ... & Sekine, Y. (2020). Acetylation-mediated remodeling of the nucleolus regulates cellular acetyl-CoA responses. PLoS biology, 18(11), e3000981.
CBMAB-XB0102-YC-4
CBMAB-XB0102-YC in WB
BOP1 protein expression in the aortic media of donors (n=4) and AD patients (n=8) was detected by western blotting, and the related expression level was detected by statistical analysis and shown. ...View More
Wu, Q., Hong, J., Wang, Z., Hu, J., Chen, R., Hu, Z., ... & Ruan, Y. (2019). Abnormal ribosome biogenesis partly induced p53-dependent aortic medial smooth muscle cell apoptosis and oxidative stress. Oxidative Medicine and Cellular Longevity, 2019.
CBMAB-XB0102-YC-5
CBMAB-XB0102-YC in IHC
Representative image of the aortic specimens stained by BOP1 and p53 by performing IHC. ...View More
Wu, Q., Hong, J., Wang, Z., Hu, J., Chen, R., Hu, Z., ... & Ruan, Y. (2019). Abnormal ribosome biogenesis partly induced p53-dependent aortic medial smooth muscle cell apoptosis and oxidative stress. Oxidative Medicine and Cellular Longevity, 2019.
CBMAB-XB0102-YC-6
CBMAB-XB0102-YC in IHC
Representative images of the aortas performed with TUNEL assays, IHC staining with anti-BOP1 antibody and anti-ki-67 antibody. ...View More
Wu, Q., Hong, J., Wang, Z., Hu, J., Chen, R., Hu, Z., ... & Ruan, Y. (2019). Abnormal ribosome biogenesis partly induced p53-dependent aortic medial smooth muscle cell apoptosis and oxidative stress. Oxidative Medicine and Cellular Longevity, 2019.
Datasheet
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
XB0102
Application
WB, IP, IF, E
Immunogen
Amino acids 120-315 mapping within an internal region of BOP1 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Block Of Proliferation 1
Introduction
BOS2 (Branchiootic Syndrome 2) is a Genetic Locus. Diseases associated with BOS2 include Branchiootic Syndrome 2 and Branchiootic Syndrome.
Entrez Gene ID
23246
UniProt ID
Q14137
Alternative Names
Block Of Proliferation 1; Block Of Proliferation 1 Protein; KIAA0124;
Function
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
Biological Process
Cell population proliferation Source: Ensembl
Cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: Ensembl
Maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB
Regulation of cell cycle Source: UniProtKB
Regulation of signal transduction by p53 class mediator Source: UniProtKB
Ribosomal large subunit assembly Source: UniProtKB
Ribosome biogenesis Source: UniProtKB
rRNA processing Source: Reactome
Cellular Location
Nucleolus; Nucleoplasm

Vellky, J. E., Ricke, E. A., Huang, W., & Ricke, W. A. (2021). Expression, Localization, and Function of the Nucleolar Protein BOP1 in Prostate Cancer Progression. The American Journal of Pathology, 191(1), 168-179.

Yang, Y. P., Qin, R. H., Zhao, J. J., & Qin, X. Y. (2021). BOP1 silencing suppresses gastric cancer proliferation through p53 modulation. Current medical science, 41(2), 287-296.

Jia, F., Wu, Q., Wang, Z., Zhang, M., Yuan, S., Che, Y., ... & Hu, X. (2021). BOP1 Knockdown Attenuates Neointimal Hyperplasia by Activating p53 and Inhibiting Nascent Protein Synthesis. Oxidative medicine and cellular longevity, 2021.

Li, S., Wu, H., Huang, X., Jian, Y., Kong, L., Xu, H., ... & Wang, X. (2021). BOP1 confers chemoresistance of triple‐negative breast cancer by promoting CBP‐mediated β‐catenin acetylation. The Journal of Pathology.

Li, W., Song, P., Zhao, M., Gao, L., Xie, J., & You, C. (2021). BOP1 Used as a Novel Prognostic Marker and Correlated with Tumor Microenvironment in Pan-Cancer. Journal of Oncology, 2021.

Chen, B., Dragomir, M. P., Fabris, L., Bayraktar, R., Knutsen, E., Liu, X., ... & Calin, G. A. (2020). The long noncoding RNA CCAT2 induces chromosomal instability through BOP1-AURKB signaling. Gastroenterology, 159(6), 2146-2162.

Gupta, R., Bugide, S., Wang, B., Green, M. R., Johnson, D. B., & Wajapeyee, N. (2019). Loss of BOP1 confers resistance to BRAF kinase inhibitors in melanoma by activating MAP kinase pathway. Proceedings of the National Academy of Sciences, 116(10), 4583-4591.

Borah, N., Nukala, K. M., Vadlapudi, V., Gubbala, S. P., & Amanchy, R. (2019). BOP1–a key player of ribosomal biogenesis. Current Science, 117(3), 422.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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