Mouse Anti-BPHL Recombinant Antibody (CBYY-0732) (CBMAB-0735-YY)

Name: Mouse Anti-BPHL Recombinant Antibody (CBYY-0732)
SKU: CBMAB-0735-YY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Mouse, Rat, Human

Clone

CBYY-0732

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, E

Basic Information

Immunogen

Amino acids 158-230 mapping within an internal region of VACVase of human origin.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

BIPHENYL HYDROLASE LIKE

Introduction

This gene encodes a member of the serine protease family of hydrolytic enzymes which contain a serine in their active site. The encoded protein may play a role in activation of the antiviral prodrug valacyclovir. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009]

Entrez Gene ID

Human	670
Mouse	68021
Rat	361239

UniProt ID

Human	Q86WA6
Mouse	Q8R164
Rat	Q3B8N9

Alternative Names

Biphenyl Hydrolase Like; Breast Epithelial Mucin-Associated Antigen; Biphenyl Hydrolase-Like (Serine Hydrolase); Biphenyl Hydrolase-Related Protein; Valacyclovir Hydrolase; Valacyclovirase;

Function

Serine hydrolase that catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. Activates valacyclovir to acyclovir. May play a role in detoxification processes. It is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol.

Biological Process

Cellular amino acid metabolic process Source: ProtInc
Response to toxic substance Source: ProtInc
Xenobiotic metabolic process Source: Reactome

Cellular Location

Mitochondrial outer membrane; Mitochondrion

More Infomation

References

Hu, Y., Epling, D., Shi, J., Song, F., Tsume, Y., Zhu, H. J., ... & Smith, D. E. (2018). Effect of biphenyl hydrolase-like (BPHL) gene disruption on the intestinal stability, permeability and absorption of valacyclovir in wildtype and Bphl knockout mice. Biochemical pharmacology, 156, 147-156.

Tao, W., Zhao, D., Sun, M., Li, M., Zhang, X., He, Z., ... & Sun, J. (2017). Enzymatic activation of double-targeted 5′-O-l-valyl-decitabine prodrug by biphenyl hydrolase-like protein and its molecular design basis. Drug delivery and translational research, 7(2), 304-311.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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