Rabbit Anti-BRIP1 Recombinant Antibody (
27F4) (V2LY-0725-LY1145)

Name: Rabbit Anti-BRIP1 Recombinant Antibody (27F4)
SKU: V2LY-0725-LY1145
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-BRIP1 Recombinant Antibody (27F4) (V2LY-0725-LY1145) in WB

Western Blot
Positive WB detected in: MCF7 whole cell lysate, HEPG2 whole cell lysate
All lanes: BACH1/BRIP1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 105 kDa
Observed band size: 105 kDa

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

27F4

Application

ELISA, WB

Immunogen

A synthesized peptide derived from Human BRIP1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

BRIP1

Entrez Gene ID

83990

UniProt ID

Q9BX63

Function

DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.

Biological Process

Cellular response to angiotensin Source: Ensembl
Cellular response to hypoxia Source: Ensembl
Cellular response to vitamin Source: Ensembl
Chiasma assembly Source: Ensembl
DNA damage checkpoint Source: UniProtKB
DNA replication Source: Reactome
Double-strand break repair Source: UniProtKB
Double-strand break repair involved in meiotic recombination Source: GO_Central
Meiotic DNA double-strand break processing involved in reciprocal meiotic recombination Source: Ensembl
Negative regulation of cell population proliferation Source: Ensembl
Negative regulation of gene expression Source: Ensembl
Nucleotide-excision repair Source: GO_Central
Regulation of signal transduction by p53 class mediator Source: Reactome
Regulation of transcription by RNA polymerase II Source: MGI
Response to toxic substance Source: Ensembl
Seminiferous tubule development Source: Ensembl
Spermatid development Source: Ensembl
Spermatogonial cell division Source: Ensembl

Cellular Location

Nucleus; Cytoplasm

Involvement in disease

Breast cancer (BC): A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Fanconi anemia complementation group J (FANCJ): A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.

PTM

Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated.
Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling.

Calvo, J. A., Fritchman, B., Hernandez, D., Persky, N. S., Johannessen, C. M., Piccioni, F., ... & Cantor, S. B. (2021). Comprehensive Mutational Analysis of the BRCA1-Associated DNA Helicase and Tumor-Suppressor FANCJ/BACH1/BRIP1. Molecular Cancer Research, 19(6), 1015-1025.

Rizeq, B., Sif, S., Nasrallah, G. K., & Ouhtit, A. (2020). Novel role of BRCA1 interacting C‐terminal helicase 1 (BRIP1) in breast tumour cell invasion. Journal of cellular and molecular medicine, 24(19), 11477-11488.

Singal, M., Godbole, M. M., Povenzano, K., Elmore, B., & Patel, M. P. (2020). Abstract P6-08-31: BRCA1 interacting protein C-terminal helicase 1 (BRIP1) as a cancer susceptibility gene.

Kamal, L., Pierce, S. B., Canavati, C., Rayyan, A. A., Jaraysa, T., Lobel, O., ... & Kanaan, M. N. (2020). Helicase-inactivating BRIP1 mutation yields Fanconi anemia with microcephaly and other congenital abnormalities. Molecular Case Studies, 6(5), a005652.

Moyer, C. L., Ivanovich, J., Gillespie, J. L., Doberstein, R., Radke, M. R., Richardson, M. E., ... & Goodfellow, P. J. (2020). Rare BRIP1 missense alleles confer risk for ovarian and breast cancer. Cancer research, 80(4), 857-867.

Ali, M., Delozier, C. D., & Chaudhary, U. (2019). BRIP-1 germline mutation and its role in colon cancer: presentation of two case reports and review of literature. BMC medical genetics, 20(1), 1-5.

Gohil, S., Barwell, J., & Levy, M. (2019, November). Arg798Ter BRIP-1 mutation associated with metastatic phaeochromocytoma. In Society for Endocrinology BES 2019 (Vol. 65). BioScientifica.

Ma, B., Wu, J., & Ma, B. (2019). Association between single nucleotide polymorphism of BRCA1-interacting protein C-terminal helicase 1 and early-onset breast cancer in Uygur and Han women in Xinjiang of China. Biotechnology & Biotechnological Equipment, 33(1), 1750-1756.

Adams, C. L., Ciccone, M. A., Gruber, S. B., & McDonnell, K. (2017). BRCA1, DNA repair associated (BRCA1)-and BRCA1 Interacting Protein C-terminal Helicase 1 (BRIP1)-Mutated Ovarian Epithelial Cells Acquire a Cancer Stem Cell-Like Phenotype and Decreased Sensitivity to Cisplatin and Olaparib in Three Dimensional Spheroid Culture. Gynecologic Oncology, 147(1), 196.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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