Mouse Anti-BRS3 Recombinant Antibody (EG417) (CBMAB-EN475-LY)

Name: Mouse Anti-BRS3 Recombinant Antibody (EG417)
SKU: CBMAB-EN475-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG417

Application

WB, IP, IF, E

Immunogen

Amino acids 27-84 mapping near the N-terminus of Bombesin Receptor Subtype-3 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Bombesin Receptor Subtype 3

Introduction

The protein encoded by this gene is a G protein-coupled membrane receptor that binds bombesin-like peptides. This binding results in activation of a phosphatidylinositol-calcium second messenger system, with physiological effects including regulation of metabolic rate, glucose metabolism, and hypertension. [provided by RefSeq, Sep 2011]

Entrez Gene ID

680

UniProt ID

P32247

Alternative Names

Bombesin Receptor Subtype 3; Bombesin Like Receptor 3; BRS-3; Bombesin Receptor Subtype-3; G-Protein Coupled Receptor; BB3R; BB3;

Function

Role in sperm cell division, maturation, or function. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Biological Process

Adult feeding behavior Source: ProtInc
Glucose metabolic process Source: ProtInc
G protein-coupled receptor signaling pathway Source: GO_Central
Regulation of blood pressure Source: ProtInc

Cellular Location

Cell membrane

Topology

Extracellular: 1-41 aa
Helical: 42-63 aa
Cytoplasmic: 64-82 aa
Helical: 83-103 aa
Extracellular: 104-121 aa
Helical: 122-143 aa
Cytoplasmic: 144-163 aa
Helical: 164-184 aa
Extracellular: 185-220 aa
Helical: 221-241 aa
Cytoplasmic: 242-272 aa
Helical: 273-293 aa
Extracellular: 294-313 aa
Helical: 314-333 aa
Cytoplasmic: 334-399 aa

Dong, L., Zhang, B., Wu, L., Shang, Z., Liu, S., Jiang, X., ... & Xiao, H. (2020). Proteomics analysis of cellular BRS3 receptor activation reveals potential mechanism for signal transduction and cell proliferation. Journal of proteome research, 19(4), 1513-1521.

Li, M., Liang, P., Liu, D., Yuan, F., Chen, G. C., Zhang, L., ... & Liu, H. (2019). Bombesin Receptor Subtype-3 in Human Diseases. Archives of medical research, 50(7), 463-467.

Zhang, Y., Liu, Y., Wu, L., Fan, C., Wang, Z., Zhang, X., ... & Civelli, O. (2018). Receptor‐specific crosstalk between prostanoid E receptor 3 and bombesin receptor subtype 3. The FASEB Journal, 32(6), 3184-3192.

Liu, H., Peng, L., Liu, C., Tan, Y., Xiang, Y., Qu, X., ... & Qin, X. (2018). Activation of bombesin receptor subtype-3 promotes antigen-presenting action in human bronchial epithelial cells. International archives of allergy and immunology, 175(1-2), 53-60.

Moreno, P., Mantey, S. A., Lee, S. H., Ramos-Álvarez, I., Moody, T. W., & Jensen, R. T. (2018). A possible new target in lung-cancer cells: The orphan receptor, bombesin receptor subtype-3. Peptides, 101, 213-226.

Moreno-Villegas, Z., Martín-Duce, A., Aparicio, C., Portal-Núñez, S., Sanz, R., Mantey, S. A., ... & González, N. (2018). Activation of bombesin receptor Subtype-3 by [D-Tyr6, β-Ala11, Phe13, Nle14] bombesin6-14 increased glucose uptake and lipogenesis in human and rat adipocytes. Molecular and cellular endocrinology, 474, 10-19.

Nio, Y., Hotta, N., Maruyama, M., Hamagami, K., Nagi, T., Funata, M., ... & Nagisa, Y. (2017). A Selective Bombesin Receptor Subtype 3 Agonist Promotes Weight Loss in Male Diet-Induced–Obese Rats With Circadian Rhythm Change. Endocrinology, 158(5), 1298-1313.

Mo, C., Huang, L., Cui, L., Lv, C., Lin, D., Song, L., ... & Wang, Y. (2017). Characterization of NMB, GRP and their receptors (BRS3, NMBR and GRPR) in chickens. Journal of molecular endocrinology, 59(1), 61-79.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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