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Mouse Anti-BTAF1 Recombinant Antibody (CBYJT-1050) (CBMAB-T0058-YJ)


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Summary

Host Animal
Mouse
Specificity
Human, Rat, Mouse
Clone
CBYJT-1050
Antibody Isotype
IgG1
Application
WB, IP, IF, FC

Basic Information

Immunogen
Recombinant protein corresponding to the C-terminal region of TAF II p170 of human origin.
Host Species
Mouse
Specificity
Human, Rat, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
B-TFIID TATA-Box Binding Protein Associated Factor 1
Introduction
BTAF1 is a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.
Entrez Gene ID
Human9044
Mouse107182
Rat368042
UniProt ID
HumanO14981
MouseE9QAE3
RatF1LW16
Alternative Names
B-TFIID TATA-Box Binding Protein Associated Factor 1; B-TFIID Transcription Factor-Associated 170 KDa Subunit; ATP-Dependent Helicase BTAF1; TBP-Associated Factor 172; TAF(II)170; TAF172; BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170 KD (Mot1 Homolog, S. Cerevisiae); BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kDa (Mot1 Homolog, S. Cerevisiae); BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kDa
Function
Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.
Biological Process
Negative regulation of chromatin binding Source: MGI
Negative regulation of transcription, DNA-templated Source: UniProtKB
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

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