What is the recommended storage condition for this recombinant antibody?

For long-term storage, it is generally recommended to store the Mouse Anti-BTN2A1 Recombinant Antibody (3A1) at -20°C or below. For short-term storage (a few weeks), it can typically be stored at 2-8°C. It is crucial to avoid repeated freeze-thaw cycles as this can damage the antibody. Aliquoting the antibody upon arrival is advisable if you plan to use it multiple times.

Is this antibody a full-length IgG molecule?

Yes, the Mouse Anti-BTN2A1 Recombinant Antibody (3A1) (CAT: CBMAB-A0907-LY) is a recombinant monoclonal antibody expressed as a full-length mouse IgG2a, kappa. Recombinant production ensures high purity and consistency compared to traditional hybridoma-derived antibodies.

Mouse Anti-BTN2A1 Recombinant Antibody (3A1) (CBMAB-A0907-LY)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3A1

Application

Immunogen

BTN2A1 (AAH16661, 1 a.a. ~ 334 a.a) full-length recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

butyrophilin, subfamily 2, member A1

Introduction

This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is an integral plasma membrane B box protein involved in lipid, fatty-acid and sterol metabolism. [provided by RefSeq]

Entrez Gene ID

11120

UniProt ID

Q7KYR7

Alternative Names

BK14H9.1; BT2.1; BTF1; DJ3E1.1; FLJ36567

Biological Process

Adaptive immune response Source: Reactome
Lipid metabolic process Source: ProtInc
Regulation of cytokine production Source: GO_Central
T cell receptor signaling pathway Source: GO_Central

Cellular Location

Membrane

Topology

Extracellular: 29-248 aa
Helical: 249-269 aa
Cytoplasmic: 270-527 aa

Cano, C. E., Pasero, C., De Gassart, A., Kerneur, C., Gabriac, M., Fullana, M., ... & Olive, D. (2021). BTN2A1, an immune checkpoint targeting Vγ9Vδ2 T cell cytotoxicity against malignant cells. Cell Reports, 36(2), 109359.

Billon, E., Chanez, B., Rochigneux, P., Albiges, L., Vicier, C., Pignot, G., ... & Olive, D. (2021). Soluble BTN2A1 Is a Potential Prognosis Biomarker in Pre-Treated Advanced Renal Cell Carcinoma. Frontiers in Immunology, 12, 1352.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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