Rabbit Anti-BUB1B Recombinant Antibody (
7H4) (V2LY-0725-LY1135)

Name: Rabbit Anti-BUB1B Recombinant Antibody (7H4)
SKU: V2LY-0725-LY1135
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-BUB1B Recombinant Antibody (7H4) (V2LY-0725-LY1135) in WB

Western Blot
Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate
All lanes: BubR1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 120, 106, 122 kDa
Observed band size: 125 kDa

Rabbit Anti-BUB1B Recombinant Antibody (7H4) (V2LY-0725-LY1135) in FC

Overlay histogram showing Hela cells stained with V2LY-0725-LY1135 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

7H4

Application

ELISA, WB, FC

Immunogen

A synthesized peptide derived from human BubR1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
FC	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

BUB1 Mitotic Checkpoint Serine/Threonine Kinase B

Entrez Gene ID

701

UniProt ID

O60566

Function

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

Biological Process

Anaphase-promoting complex-dependent catabolic process Source: Reactome
Apoptotic process Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Meiotic sister chromatid cohesion, centromeric Source: GO_Central
Metaphase/anaphase transition of mitotic cell cycle Source: Ensembl
Mitotic cell cycle Source: ProtInc
Mitotic cell cycle checkpoint Source: ProtInc
Mitotic spindle assembly checkpoint Source: GO_Central
Mitotic spindle organization Source: Reactome
Protein localization to chromosome, centromeric region Source: Ensembl
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome

Cellular Location

Cytoplasm; Nucleus; Centrosome; Kinetochore. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and KNL1.

Involvement in disease

Defects in BUB1B are associated with tumor formation.
Premature chromatid separation trait (PCS): Consists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant.
Mosaic variegated aneuploidy syndrome 1 (MVA1): A severe developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases.

PTM

Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.
Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.
Ubiquitinated. Degraded by the proteasome.
Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.
Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 ^oCcurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.

Mamoor, S. (2021). Over-expression of BUB1 mitotic checkpoint serine/threonine kinase B in human endometrial cancer.

Mamoor, S. (2021). Differential expression of BUB1 mitotic checkpoint serine/threonine kinase B in human epithelial ovarian cancer.

Mamoor, S. (2021). Differential expression of BUB1B in cancers of the breast.

Long, Z., Wu, T., Tian, Q., Carlson, L. A., Wang, W., & Wu, G. (2021). Expression and prognosis analyses of BUB1, BUB1B and BUB3 in human sarcoma. Aging (Albany NY), 13(9), 12395.

Sekino, Y., Han, X., Kobayashi, G., Babasaki, T., Miyamoto, S., Kobatake, K., ... & Matsubara, A. (2021). BUB1B Overexpression Is an Independent Prognostic Marker and Associated with CD44, p53, and PD-L1 in Renal Cell Carcinoma. Oncology, 99(4), 240-250.

Qiu, J., Zhang, S., Wang, P., Wang, H., Sha, B., Peng, H., ... & Lu, L. (2020). BUB1B promotes hepatocellular carcinoma progression via activation of the mTORC1 signaling pathway. Cancer medicine, 9(21), 8159-8172.

Tian, X., & Wang, N. (2020). Upregulation of ASPM, BUB1B and SPDL1 in tumor tissues predicts poor survival in patients with pancreatic ductal adenocarcinoma. Oncology letters, 19(4), 3307-3315.

Yang, W. X., Pan, Y. Y., & You, C. G. (2019). CDK1, CCNB1, CDC20, BUB1, MAD2L1, MCM3, BUB1B, MCM2, and RFC4 may be potential therapeutic targets for hepatocellular carcinoma using integrated bioinformatic analysis. BioMed research international, 2019.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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