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Mouse Anti-RNF213 Recombinant Antibody (5C12) (CBMAB-A0940-LY)

The product is antibody recognizes C17orf27. The antibody 5C12 immunoassay techniques such as: WB, ELISA.
See all RNF213 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5C12
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
C17orf27 (NP_065965, 2016 a.a. ~ 2112 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Ring Finger Protein 213
Entrez Gene ID
57674
UniProt ID
Q63HN8
Alternative Names
C17orf27; KIAA1554
Function
Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (PubMed:21799892, PubMed:26126547, PubMed:26278786, PubMed:26766444, PubMed:30705059, PubMed:32139119, PubMed:34012115).
Acts as a key immune sensor by catalyzing ubiquitination of the lipid A moiety of bacterial lipopolysaccharide (LPS) via its RZ-type zinc-finger: restricts the proliferation of cytosolic bacteria, such as Salmonella, by generating the bacterial ubiquitin coat through the ubiquitination of LPS (PubMed:34012115).
Also acts indirectly by mediating the recruitment of the LUBAC complex, which conjugates linear polyubiquitin chains (PubMed:34012115).
Ubiquitination of LPS triggers cell-autonomous immunity, such as antibacterial autophagy, leading to degradation of the microbial invader (PubMed:34012115).
Involved in lipid metabolism by regulating fat storage and lipid droplet formation; act by inhibiting the lipolytic process (PubMed:30705059).
Also regulates lipotoxicity by inhibiting desaturation of fatty acids (PubMed:30846318).
Also acts as an E3 ubiquitin-protein ligase via its RING-type zinc finger: mediates 'Lys-63'-linked ubiquitination of target proteins (PubMed:32139119, PubMed:33842849).
Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression (PubMed:26766444).
Also has ATPase activity; ATPase activity is required for ubiquitination of LPS (PubMed:34012115).
Biological Process
Biological Process angiogenesisManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process defense response to bacteriumManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process immune system processIEA:UniProtKB-KW
Biological Process lipid droplet formationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process lipid ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process negative regulation of non-canonical Wnt signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein autoubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein K63-linked ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of lipid metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process sprouting angiogenesisManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process xenophagyManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm, cytosol
Lipid droplet
Involvement in disease
Moyamoya disease 2 (MYMY2):
A progressive cerebral angiopathy characterized by bilateral intracranial carotid artery stenosis and telangiectatic vessels in the region of the basal ganglia. The abnormal vessels resemble a 'puff of smoke' (moyamoya) on cerebral angiogram. Affected individuals can develop transient ischemic attacks and/or cerebral infarction, and rupture of the collateral vessels can cause intracranial hemorrhage. Hemiplegia of sudden onset and epileptic seizures constitute the prevailing presentation in childhood, while subarachnoid bleeding occurs more frequently in adults.
PTM
Autoubiquitinated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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